Cloned (Comment) | Organism |
---|---|
gene TmGlu1, DNA and amino acid sequeence determination and analysis, recombinant secretory expression of the enzyme in Pichia pastoris strain X-33 | Tricholoma matsutake |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition | Tricholoma matsutake | |
Cu2+ | 14% inhibition at 5 mM | Tricholoma matsutake | |
Fe2+ | 48% inhibition at 5 mM | Tricholoma matsutake | |
Hg2+ | 52% inhibition at 5 mM | Tricholoma matsutake | |
Pb2+ | 30% inhibition at 5 mM | Tricholoma matsutake | |
Zn2+ | 27% inhibition at 5 mM | Tricholoma matsutake |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Tricholoma matsutake | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 29% activation at 5 mM | Tricholoma matsutake | |
DTT | 25% activation at 5 mM | Tricholoma matsutake | |
Mn2+ | slight activation at 5 mM | Tricholoma matsutake | |
additional information | Ba2+, K+, Al3+, Mg2+, Fe3+, Na+, Ni+, Li+, Co2+, and EDTA have poor effects on activity | Tricholoma matsutake |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63900 | - |
gel filtration | Tricholoma matsutake |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Tricholoma matsutake | soluble starch | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tricholoma matsutake | H1AAU7 | - |
- |
Tricholoma matsutake NBRC 30605 | H1AAU7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Tricholoma matsutake |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 33.2fold by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Tricholoma matsutake |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | high glucoamylase activity is induced upon culture of the fungus in a medium containing amylose compared with other saccharides such as glucose, rice starch, wheat starch, corn starch, and potato starch | Tricholoma matsutake | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.2 | - |
purified enzyme, pH 5.0, 60°C, with soluble starch as substrate | Tricholoma matsutake |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Tricholoma matsutake | ? | - |
? | |
amylose A + H2O | - |
Tricholoma matsutake | ? | - |
? | |
amylose B + H2O | - |
Tricholoma matsutake | ? | - |
? | |
glycogen + H2O | - |
Tricholoma matsutake | ? | - |
? | |
isomaltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
maltopentaose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
maltopentaose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
maltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
maltotetraose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
maltotetraose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
maltotriose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
maltotriose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake | ? | - |
? | |
additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake NBRC 30605 | ? | - |
? | |
pullulan + H2O | - |
Tricholoma matsutake | ? | - |
? | |
pullulan + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
starch + H2O | soluble starch | Tricholoma matsutake | ? | - |
? | |
starch + H2O | soluble starch, and low activity with raw corn starch | Tricholoma matsutake | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 61500, SDS-PAGE | Tricholoma matsutake |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Tricholoma matsutake |
TmGLA | - |
Tricholoma matsutake |
TmGlu1 | - |
Tricholoma matsutake |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Tricholoma matsutake |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | activity range, profile overview | Tricholoma matsutake |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | purified enzyme, stable at | Tricholoma matsutake |
70 | - |
purified enzyme, inactivation | Tricholoma matsutake |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme | Tricholoma matsutake |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6.5 | activity range, profile overview | Tricholoma matsutake |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
3 | - |
purified enzyme, loss of 80% activity | Tricholoma matsutake |
4 | 6 | purified enzyme, stable at | Tricholoma matsutake |
7 | - |
purified enzyme, loss of 60% activity | Tricholoma matsutake |
8 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 15, GH15 | Tricholoma matsutake |