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Literature summary for 3.2.1.31 extracted from

  • Schulz, M.; Weissenböck, G.
    Partial purification and characterization of luteolin-triglucuronide-specific beta-glucuronidase from rye primary leaves (Secale cereale) (1987), Phytochemistry, 26, 933-937.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ethyleneglycol activation Secale cereale

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Secale cereale
Cu2+
-
Secale cereale
D-saccharic acid 1,4-lactone
-
Secale cereale
methanol
-
Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
luteolin 7-O-diglucuronide 4'-O-glucuronide
-
Secale cereale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
luteolin triglucuronide + H2O Secale cereale
-
?
-
?

Organism

Organism UniProt Comment Textmining
Secale cereale
-
rye
-

Purification (Commentary)

Purification (Comment) Organism
partial Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Secale cereale

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, at least 2 months Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apigenin 7,4'-diglucuronide + H2O
-
Secale cereale apigenin 7-O-glucuronide + glucuronic acid
-
?
luteolin 7-O-glucuronide + H2O
-
Secale cereale ?
-
?
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] + H2O
-
Secale cereale ?
-
?
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O
-
Secale cereale ?
-
?
luteolin triglucuronide + H2O
-
Secale cereale ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Secale cereale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
denaturation above Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
2.1 5.8 half maximal activity Secale cereale