Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L527I/M543I | 158% increased activity compared to the wild type enzyme | Escherichia coli |
S193N/G466A/Q951R | 212% increased activity compared to the wild type enzyme | Escherichia coli |
S193N/T266A/Q267R/Q626R | 167% increased activity compared to the wild type enzyme | Escherichia coli |
S193N/V411A/D448G | 172% increased activity compared to the wild type enzyme | Escherichia coli |
T266A/Q267R/Q626R | 137% increased activity compared to the wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel chelate affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
o-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli | o-nitrophenol + D-glucuronic acid | - |
? | |
p-nitrophenyl-beta-D-glucuronide + H2O | - |
Escherichia coli | p-nitrophenol + D-glucuronic acid | - |
? |