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Literature summary for 3.2.1.32 extracted from

  • Chen, W.P.; Matsuo, M.; Yasui, T.
    Purification and some properties of beta-1,3-xylanase from Aspergillus terreus A-07 (1986), Agric. Biol. Chem., 50, 1183-1194.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, strong inhibition Aspergillus terreus
Mn2+ 1 mM, strong inhibition Aspergillus terreus
N-bromosuccinimide 1 mM, strong inhibition Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
A-07
-
Aspergillus terreus A-07
-
A-07
-

Purification (Commentary)

Purification (Comment) Organism
6 different enzyme forms: EF-1, EF-2, EF-3, EF-4, EF-5 and EF-6 Aspergillus terreus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
enzyme form EF-3 and EF-5 Aspergillus terreus
8.5
-
enzyme form EF-4 Aspergillus terreus
9.3
-
enzyme form EF-2 Aspergillus terreus
13.3
-
enzyme form EF-6 Aspergillus terreus
15.2
-
enzyme form EF-1 Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose Aspergillus terreus xylose + xylooligosaccharides
-
?
beta-1,3-xylan + H2O action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose Aspergillus terreus A-07 xylose + xylooligosaccharides
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
enzyme form EF-4 and EF-5 Aspergillus terreus
45
-
enzyme form EF-2 Aspergillus terreus
50
-
enzyme form EF-3 and EF-6 Aspergillus terreus
55
-
enzyme form EF-1 Aspergillus terreus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 4.5, 1 h, enzyme forms EF-5 and EF-6, stable up to Aspergillus terreus
60
-
pH 4.5, 1 h, enzyme forms EF-1, EF-2, EF-3 and EF-4 are stable, enzyme form EF-5 and EF-6 rapidly lose activity Aspergillus terreus
70
-
pH 4.5, 1 h, rapid decrease of activity of enzyme forms EF-1, EF-2, EF-3 and EF-4 Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
enzyme form EF-4 Aspergillus terreus
4.5
-
enzyme forms EF-5 and EF-6 Aspergillus terreus
5
-
enzyme forms EF-1 and EF-3 Aspergillus terreus
5.5
-
enzyme form EF-2 Aspergillus terreus