Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, strong inhibition | Aspergillus terreus | |
Mn2+ | 1 mM, strong inhibition | Aspergillus terreus | |
N-bromosuccinimide | 1 mM, strong inhibition | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
A-07 | - |
Aspergillus terreus A-07 | - |
A-07 | - |
Purification (Comment) | Organism |
---|---|
6 different enzyme forms: EF-1, EF-2, EF-3, EF-4, EF-5 and EF-6 | Aspergillus terreus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.4 | - |
enzyme form EF-3 and EF-5 | Aspergillus terreus |
8.5 | - |
enzyme form EF-4 | Aspergillus terreus |
9.3 | - |
enzyme form EF-2 | Aspergillus terreus |
13.3 | - |
enzyme form EF-6 | Aspergillus terreus |
15.2 | - |
enzyme form EF-1 | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-xylan + H2O | action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose | Aspergillus terreus | xylose + xylooligosaccharides | - |
? | |
beta-1,3-xylan + H2O | action pattern of type I occurs in the hydrolysis by enzyme form EF-1, EF-2 and EF-5: beta-1,3-xylobiose, beta-1,3-xylotriose, beta-1,3-xylotetraose and an unknown disaccharide are liberated. The action pattern of type II occurs during hydrolysis by enzyme form EF-4 and EF-6. In the initial stage the enzyme produces mainly beta-1,2-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose. As hydrolysis proceeds an isomeric xylotriose is detectable as well as xylose, beta-1,3-xylobiose, beta-1,3-xylotriose and beta-1,3-xylotetraose | Aspergillus terreus A-07 | xylose + xylooligosaccharides | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
enzyme form EF-4 and EF-5 | Aspergillus terreus |
45 | - |
enzyme form EF-2 | Aspergillus terreus |
50 | - |
enzyme form EF-3 and EF-6 | Aspergillus terreus |
55 | - |
enzyme form EF-1 | Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 4.5, 1 h, enzyme forms EF-5 and EF-6, stable up to | Aspergillus terreus |
60 | - |
pH 4.5, 1 h, enzyme forms EF-1, EF-2, EF-3 and EF-4 are stable, enzyme form EF-5 and EF-6 rapidly lose activity | Aspergillus terreus |
70 | - |
pH 4.5, 1 h, rapid decrease of activity of enzyme forms EF-1, EF-2, EF-3 and EF-4 | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
enzyme form EF-4 | Aspergillus terreus |
4.5 | - |
enzyme forms EF-5 and EF-6 | Aspergillus terreus |
5 | - |
enzyme forms EF-1 and EF-3 | Aspergillus terreus |
5.5 | - |
enzyme form EF-2 | Aspergillus terreus |