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Literature summary for 3.2.1.32 extracted from

  • Liang, W.; Fang, T.; Lin, H.; Liu, T.; Lu, W.; Tzou, W.; Tang, S.; Lin, F.; Liu, S.; Pan, C.
    Cloning, expression, and characterization of Pseudomonas vesicularis MA103 beta-1,3-xylanase in Escherichia coli ClearColi BL21(DE3) (2015), Fish. Sci., 81, 1135-1143 .
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Search for more literature for 3.2.1.32

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET-39b(+) expression vector and transformed into Escherichia coli ClearColi BL21(DE3) Brevundimonas vesicularis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 95% loss of activity Brevundimonas vesicularis
Ca2+ 1 mM, 7% loss of activity. 10 mM, 18% loss of activity Brevundimonas vesicularis
Cd2+ 1 mM, activation to 103% of control. 10 mM, 28% loss of activity Brevundimonas vesicularis
Co2+ 1 mM, activation to 102% of control. 10 mM, 71% loss of activity Brevundimonas vesicularis
Cs+ 5 mM, 4% loss of activity. 10 mM, 6% loss of activity Brevundimonas vesicularis
Fe2+ 1 mM, activation to 104% of control. 10 mM, 31% loss of activity Brevundimonas vesicularis
Hg2+ 1 mM, 60% loss of activity. 10 mM, 74% loss of activity Brevundimonas vesicularis
K+ 1 mM, 20% loss of activity. 10 mM, 19% loss of activity Brevundimonas vesicularis
Mg2+ 10 mM, 10% loss of activity Brevundimonas vesicularis
Sr2+ 1 mM, activation to 108% of control. 10 mM, 4% loss of activity Brevundimonas vesicularis
Zn2+ 1 mM, 9% loss of activity. 10 mM, 62% loss of activity Brevundimonas vesicularis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 1 mM, activation to 103% of control. 10 mM, 28% loss of activity Brevundimonas vesicularis
Co2+ 1 mM, activation to 102% of control. 10 mM, 71% loss of activity Brevundimonas vesicularis
Cu2+ 10 mM, activation to 114% of control Brevundimonas vesicularis
Fe2+ 1 mM, activation to 104% of control. 10 mM, 31% loss of activity Brevundimonas vesicularis
Mn2+ 1 mM, activation to 110% of control. 10 mM, activation to 122% of control Brevundimonas vesicularis
Na+ 1 mM, activation to 102% of control. 10 mM, activation to 106% of control Brevundimonas vesicularis
Sr2+ 1 mM, activation to 108% of control. 10 mM, 4% loss of activity Brevundimonas vesicularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63168
-
 calculated from sequence Brevundimonas vesicularis
91000
-
 SDS-PAGE, recombinant enzyme Brevundimonas vesicularis

Organism

Organism UniProt Comment Textmining
Brevundimonas vesicularis A0A0A1C3U6
-
-
Brevundimonas vesicularis MA103 A0A0A1C3U6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brevundimonas vesicularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-xylan + H2O
-
 Brevundimonas vesicularis ?
-
 ?
beta-1,3-xylan + H2O
-
 Brevundimonas vesicularis MA103 ?
-
 ?

Synonyms

Synonyms Comment Organism
xylII
-
 Brevundimonas vesicularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Brevundimonas vesicularis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 40 35°C: optimum, 40°C: 82% of maximal activity Brevundimonas vesicularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 35 stable at 0-35°C for 1 h Brevundimonas vesicularis
60
-
 1 h, complete loss of activity Brevundimonas vesicularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Brevundimonas vesicularis

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: 32% of maximal activity, pH 9.0: 40% of maximal activity Brevundimonas vesicularis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 1 h, 35°C, the enzyme remains stable at pH 6.0-8.0 with more than 90 % of activity Brevundimonas vesicularis