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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of an extracellular beta-xylosidase of Termitomyces clypeatus (1997), Biotechnol. Prog., 13, 822-827.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| improved pH- and temperature-stability as native sucrase-cellobiase-xylosidase aggregate | Termitomyces clypeatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | total inhibition at 10 mM | Termitomyces clypeatus |  |
| beta-D-xylose | 50% inhibition at 10 mM | Termitomyces clypeatus | |
| Cu2+ | total inhibition at 10 mM | Termitomyces clypeatus | |
| Fe2+ | total inhibition at 10 mM | Termitomyces clypeatus | |
| guanidinium hydrochloride | 50% inhibition at 3 M | Termitomyces clypeatus | |
| SDS | 100% inhibition at 0.2%, w/v | Termitomyces clypeatus | |
| Urea | 100% inhibition at 3 M | Termitomyces clypeatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
p-nitrophenyl beta-D-xyloside | - |
Termitomyces clypeatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Termitomyces clypeatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
SDS-PAGE, gel filtration | Termitomyces clypeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylo-oligosaccharides + H2O | Termitomyces clypeatus | derived from xylan | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Termitomyces clypeatus | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Termitomyces clypeatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 4step chromatography techniques | Termitomyces clypeatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | highest enzyme production in xylan medium | Termitomyces clypeatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 94 | - |
- |
Termitomyces clypeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl-beta-D-xyloside + H2O | - |
Termitomyces clypeatus | beta-D-xylose + methanol | - |
? | |
| p-nitrophenyl beta-D-xyloside + H2O | - |
Termitomyces clypeatus | p-nitrophenol + beta-D-xylose | - |
? | |
| xylo-oligosaccharides + H2O | derived from xylan | Termitomyces clypeatus | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | - |
Termitomyces clypeatus | beta-D-xylose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 94000, SDS-PAGE, gel filtration | Termitomyces clypeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Termitomyces clypeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Termitomyces clypeatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
guanidinium hydrochloride | - |
Termitomyces clypeatus | |
| 10 | - |
beta-D-xylose | - |
Termitomyces clypeatus | |
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