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Literature summary for 3.2.1.37 extracted from
- Genetic organization, sequence and biochemical characterzation of recombinant beta-xylosidase from Thermoanaerobacterium saccharolyticumm strain B6A-RI (1993), J. Gen. Microbiol., 139, 1235-1243.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE, calculation from sequence of cDNA | Thermoanaerobacterium saccharolyticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylo-oligosaccharides + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + ? | - |
? |  |
| xylobiose + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium saccharolyticum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by heat treatment and 2step chromatography techniques | Thermoanaerobacterium saccharolyticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.53 | - |
- |
Thermoanaerobacterium saccharolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacterium saccharolyticum | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | high specificity, transxylosylation activity | Thermoanaerobacterium saccharolyticum | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylo-oligosaccharides + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + D-xylose | - |
? | |
| xylopentaose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
| xylotetraose + H2O | - |
Thermoanaerobacterium saccharolyticum | xylotriose + beta-D-xylose | - |
? | |
| xylotriose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + xylobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable up to, loss of activity at 85°C | Thermoanaerobacterium saccharolyticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.6 | high activity within | Thermoanaerobacterium saccharolyticum |
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