Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Acetate buffer | increasing activity | Aureobasidium pullulans | |
dithiothreitol | increasing activity at 100 mM | Aureobasidium pullulans |
General Stability | Organism |
---|---|
citrate phosphate and piperazine-HCl buffers reduce activity | Aureobasidium pullulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inactivation at 100 mM | Aureobasidium pullulans | |
SDS | slight inhibition at 0.1%, w/v | Aureobasidium pullulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
p-nitrophenyl beta-D-xyloside | - |
Aureobasidium pullulans | |
17.5 | - |
xylobiose | - |
Aureobasidium pullulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aureobasidium pullulans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
121000 | - |
2 * 121000, SDS-PAGE | Aureobasidium pullulans |
224000 | - |
gel filtration, SDS-PAGE | Aureobasidium pullulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylobiose + H2O | Aureobasidium pullulans | - |
beta-D-xylose + D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aureobasidium pullulans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylated with high mannose structure | Aureobasidium pullulans |
Purification (Comment) | Organism |
---|---|
to homogeneity by 4step chromatography techniques | Aureobasidium pullulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | highest enzyme production in xylan medium | Aureobasidium pullulans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Aureobasidium pullulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl beta-D-xyloside + H2O | - |
Aureobasidium pullulans | p-nitrophenol + beta-D-xylose | - |
? | |
xylobiose + H2O | - |
Aureobasidium pullulans | beta-D-xylose + D-xylose | - |
? | |
xylotriose + H2O | also transxylosylation activity to xylotetraose | Aureobasidium pullulans | beta-D-xylose + xylobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 121000, SDS-PAGE | Aureobasidium pullulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Aureobasidium pullulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
27% loss of activity after 60 min | Aureobasidium pullulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aureobasidium pullulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9.5 | stable within | Aureobasidium pullulans |