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Literature summary for 3.2.1.37 extracted from
- 1,4-beta-D-Xylan xylohydrolase of Sclerotium rolfsii (1988), Methods Enzymol., 160, 679-684.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| no loss of activity if stored at -15°C at pH 4.5 | Athelia rolfsii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 78% inhibition at 2 mM | Athelia rolfsii |  |
| beta-D-xylose | 8-15% inhibition at 1-3 mM | Athelia rolfsii | |
| Cu2+ | 30% inhibition at 2 mM | Athelia rolfsii | |
| Hg2+ | complete inhibition at 2 mM | Athelia rolfsii | |
| iodoacetamide | highly inhibitory | Athelia rolfsii | |
| N-bromosuccinimide | highly inhibitory at 1-2 mM | Athelia rolfsii | |
| p-hydroxymercuribenzoate | highly inhibitory at 1-2 mM | Athelia rolfsii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Athelia rolfsii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Athelia rolfsii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | 180000 | gel filtration, SDS-PAGE | Athelia rolfsii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | Athelia rolfsii | traces of xylanase activity | beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | Athelia rolfsii | - |
beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Athelia rolfsii | transxylosylation activity | beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Athelia rolfsii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 2step chromatography, isoelectric focusing | Athelia rolfsii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Athelia rolfsii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.25 | - |
- |
Athelia rolfsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Athelia rolfsii | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Athelia rolfsii | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylan + H2O | traces of xylanase activity | Athelia rolfsii | beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | - |
Athelia rolfsii | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | transxylosylation activity | Athelia rolfsii | beta-D-xylose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 170000-180000, gel filtration, SDS-PAGE | Athelia rolfsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Athelia rolfsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
retains 92% of its activity for 90 min | Athelia rolfsii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Athelia rolfsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | stable within, not active below pH 2.5 or above pH 6.5 | Athelia rolfsii |
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