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Literature summary for 3.2.1.37 extracted from
- Characterization of a beta-xylosidase from Clostridium clariflavum and its application in xylan hydrolysis (2017), BioResources, 12, 9253-9262 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Acetivibrio clariflavus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-xylose | - |
Acetivibrio clariflavus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.97 | - |
xylobiose | at pH 6.0 and 60°C | Acetivibrio clariflavus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio clariflavus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Acetivibrio clariflavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Acetivibrio clariflavus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| corncob xylan + H2O | - |
Acetivibrio clariflavus | D-xylose + ? | - |
? | |
| xylobiose + H2O | the enzyme is highly active on xylobiose | Acetivibrio clariflavus | 2 D-xylose | - |
? | |
| xylotetraose + H2O | - |
Acetivibrio clariflavus | xylotriose + D-xylose | - |
? | |
| xylotriose + H2O | - |
Acetivibrio clariflavus | xylobiose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, recombinant enzyme, SDS-PAGE | Acetivibrio clariflavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylosidase | - |
Acetivibrio clariflavus |
| Xyl43C | - |
Acetivibrio clariflavus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acetivibrio clariflavus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | about 45% activity at 40°C, about 70% activity at 50°C, about 85% activity at 55°C, 100% activity at 60°C, about 90% activity at 65°C, about 45% activity at 70°C | Acetivibrio clariflavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | the enzyme retains 94.4% activity after incubation at 55°C for 1 h and 75.4% activity at 60°C for 1 h | Acetivibrio clariflavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Acetivibrio clariflavus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 100 | - |
at pH 6.0 and 60°C | Acetivibrio clariflavus | D-xylose | |
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