Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.37 extracted from

  • Yuan, Y.; Hu, Y.; Zhang, H.; Leng, J.; Li, F.; Zhao, X.; Gao, J.; Zhou, Y.
    Characterization of a recombinant multifunctional glycoside hydrolase family 3 beta-xylosidase/alpha-L-arabinofuranosidase/beta-glucosidase from Cellulosimicrobium cellulans sp. 21 (2016), J. Mol. Catal. B, 131, 65-72 .
No PubMed abstract available
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cellulosimicrobium cellulans

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 31.8% residual activity at 50 mM Cellulosimicrobium cellulans
Ca2+ 20.3% residual activity at 50 mM Cellulosimicrobium cellulans
Cu2+ 0.4% residual activity at 50 mM Cellulosimicrobium cellulans
dithiothreitol 49.2% residual activity at 50 mM Cellulosimicrobium cellulans
EDTA 1.2% residual activity at 50 mM Cellulosimicrobium cellulans
Fe3+ complete inhibition at 50 mM Cellulosimicrobium cellulans
Hg2+ complete inhibition at 50 mM Cellulosimicrobium cellulans
Mn2+ 16% residual activity at 50 mM Cellulosimicrobium cellulans
SDS complete inhibition at 50 mM Cellulosimicrobium cellulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 4-nitrophenyl beta-D-xyloside at pH 8.0 and 37°C Cellulosimicrobium cellulans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 132.2% activity at 50 mM Cellulosimicrobium cellulans
Mg2+ 108% activity at 50 mM Cellulosimicrobium cellulans
Na+ 132.6% activity at 50 mM Cellulosimicrobium cellulans

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium cellulans
-
-
-
Cellulosimicrobium cellulans 21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Cellulosimicrobium cellulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucoside + H2O 6.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 2-nitrophenol + D-glucose
-
 ?
2-nitrophenyl beta-D-glucoside + H2O 6.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 2-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-galactoside + H2O 0.3% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl alpha-D-glucoside + H2O 1.5% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucoside + H2O 1.5% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-mannoside + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + mannose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O 14.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O 1.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O 1.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O 9.6% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O 9.6% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-xyloside + H2O 100% activity Cellulosimicrobium cellulans 4-nitrophenol + D-xylose
-
 ?
laminaribose + H2O 17.5% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
laminaritriose + H2O 14.4% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
additional information no activity with gentiobiose, cellobiose, and cellotriose Cellulosimicrobium cellulans ?
-
 ?
additional information no activity with gentiobiose, cellobiose, and cellotriose Cellulosimicrobium cellulans 21 ?
-
 ?
sophorose + H2O 19.9% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
xylobiose + H2O 100% Cellulosimicrobium cellulans 2 D-xylose
-
 ?
xylotriose + H2O 100% activity compared to xylobiose Cellulosimicrobium cellulans xylobiose + D-xylose
-
 ?

Subunits

Subunits Comment Organism
? x * 95000, SDS-PAGE Cellulosimicrobium cellulans

Synonyms

Synonyms Comment Organism
ccxyl3a
-
 Cellulosimicrobium cellulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cellulosimicrobium cellulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme remains stable for 1 h up to 40°C Cellulosimicrobium cellulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.4
-
 4-nitrophenyl beta-D-xyloside at pH 8.0 and 37°C Cellulosimicrobium cellulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Cellulosimicrobium cellulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.5 the enzyme retains more than 60% activity between pH 6.5 and 9.5 for 24 h Cellulosimicrobium cellulans