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Literature summary for 3.2.1.37 extracted from

  • Bankeeree, W.; Akada, R.; Lotrakul, P.; Punnapayak, H.; Prasongsuk, S.
    Enzymatic hydrolysis of black liquor xylan by a novel xylose-tolerant, thermostable beta-xylosidase from a tropical strain of Aureobasidium pullulans CBS 135684 (2018), Appl. Biochem. Biotechnol., 184, 919-934 .
    View publication on PubMed

Application

Application Comment Organism
additional information the beta-xylosidase from Aureobasidium pullulans CBS 135684 is a prospective enzyme for a wide range of applications, especially for the bioconversion of hemicellulose Aureobasidium pullulans

Inhibitors

Inhibitors Comment Organism Structure
D-xylose tolerant to xylose inhibition with a Ki value of 18 mM Aureobasidium pullulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.57
-
4-nitrophenyl beta-D-xyloside 70°C, pH 6.0 Aureobasidium pullulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
172000
-
SDS-PAGE Aureobasidium pullulans

Organism

Organism UniProt Comment Textmining
Aureobasidium pullulans
-
-
-
Aureobasidium pullulans CBS 135684
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aureobasidium pullulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xyloside + H2O the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose Aureobasidium pullulans 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xyloside + H2O the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose Aureobasidium pullulans CBS 135684 4-nitrophenol + beta-D-xylose
-
?
xylan + H2O when combining this beta-xylosidase with xylanase from the same Aureobasidium pullulans strain, the rate of black liquor xylan hydrolysis is significantly improved by up to 1.6fold Aureobasidium pullulans beta-D-xylose + ?
-
?
xylan + H2O when combining this beta-xylosidase with xylanase from the same Aureobasidium pullulans strain, the rate of black liquor xylan hydrolysis is significantly improved by up to 1.6fold Aureobasidium pullulans CBS 135684 beta-D-xylose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Aureobasidium pullulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
12 h, enzyme retains 80% of its initial activity. Half-life: 38 h Aureobasidium pullulans
65
-
half-life: 22 h Aureobasidium pullulans
70
-
half-life: 10 h Aureobasidium pullulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aureobasidium pullulans

pH Range

pH Minimum pH Maximum Comment Organism
5 9 active over a relatively wide pH range (5.0-9.0) with more than 80% of the maximum activity Aureobasidium pullulans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
18
-
D-xylose 70°C, pH 6.0 Aureobasidium pullulans