Application | Comment | Organism |
---|---|---|
additional information | the beta-xylosidase from Aureobasidium pullulans CBS 135684 is a prospective enzyme for a wide range of applications, especially for the bioconversion of hemicellulose | Aureobasidium pullulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-xylose | tolerant to xylose inhibition with a Ki value of 18 mM | Aureobasidium pullulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.57 | - |
4-nitrophenyl beta-D-xyloside | 70°C, pH 6.0 | Aureobasidium pullulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
172000 | - |
SDS-PAGE | Aureobasidium pullulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aureobasidium pullulans | - |
- |
- |
Aureobasidium pullulans CBS 135684 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aureobasidium pullulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xyloside + H2O | the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose | Aureobasidium pullulans | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xyloside + H2O | the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose | Aureobasidium pullulans CBS 135684 | 4-nitrophenol + beta-D-xylose | - |
? | |
xylan + H2O | when combining this beta-xylosidase with xylanase from the same Aureobasidium pullulans strain, the rate of black liquor xylan hydrolysis is significantly improved by up to 1.6fold | Aureobasidium pullulans | beta-D-xylose + ? | - |
? | |
xylan + H2O | when combining this beta-xylosidase with xylanase from the same Aureobasidium pullulans strain, the rate of black liquor xylan hydrolysis is significantly improved by up to 1.6fold | Aureobasidium pullulans CBS 135684 | beta-D-xylose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aureobasidium pullulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
12 h, enzyme retains 80% of its initial activity. Half-life: 38 h | Aureobasidium pullulans |
65 | - |
half-life: 22 h | Aureobasidium pullulans |
70 | - |
half-life: 10 h | Aureobasidium pullulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aureobasidium pullulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | active over a relatively wide pH range (5.0-9.0) with more than 80% of the maximum activity | Aureobasidium pullulans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
D-xylose | 70°C, pH 6.0 | Aureobasidium pullulans |