Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Talaromyces amestolkiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | about 50% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
Cu2+ | about 75% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
D-xylose | product inhibition | Talaromyces amestolkiae | |
Hg2+ | about 10% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
additional information | absence of inhibition in the presence of EDTA, dithiothreitol, and 2-mercaptoethanol | Talaromyces amestolkiae | |
Ni2+ | about 75% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
Pb2+ | about 90% residual activity at 5 mM in citrate buffer | Talaromyces amestolkiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
0.2 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
0.2 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
0.22 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
0.22 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
0.48 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.6 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
5.8 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | about 120% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
Co2+ | about 110% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
Fe2+ | about 125% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
Mg2+ | about 110% activity at 5 mM in acetate buffer | Talaromyces amestolkiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Talaromyces amestolkiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces amestolkiae | A0A0H3W535 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Talaromyces amestolkiae |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography, Mono S column chromatography, and Superose 12 gel filtration | Talaromyces amestolkiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Talaromyces amestolkiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + L-arabinopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + xylobiose | - |
Talaromyces amestolkiae | 4-nitrophenol + xylotriose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + xylotriose | - |
Talaromyces amestolkiae | 4-nitrophenol + xylotetraose | - |
? | |
beechwood xylan + H2O | - |
Talaromyces amestolkiae | D-xylose + ? | - |
? | |
additional information | no activity with 4-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-fucopyranoside | Talaromyces amestolkiae | ? | - |
? | |
xylan + H2O | - |
Talaromyces amestolkiae | ? | - |
? | |
xylobiose + H2O | - |
Talaromyces amestolkiae | 2 D-xylose | - |
? | |
xylohexaose + H2O | - |
Talaromyces amestolkiae | 6 D-xylose | - |
? | |
xylopentaose + H2O | - |
Talaromyces amestolkiae | 5 D-xylose | - |
? | |
xylotetraose + 3 H2O | - |
Talaromyces amestolkiae | 4 D-xylose | - |
? | |
xylotriose + H2O | - |
Talaromyces amestolkiae | 3 D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 100000, SDS-PAGE | Talaromyces amestolkiae |
homodimer | 2 * 102275, MALDI-TOF mass spectrometry | Talaromyces amestolkiae |
homodimer | 2 * 84374, calculated from amino acid sequence | Talaromyces amestolkiae |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase | - |
Talaromyces amestolkiae |
BxTW1 | - |
Talaromyces amestolkiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Talaromyces amestolkiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | more than 50% activity between 50 and 70°C | Talaromyces amestolkiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme retains more than 70% activity and more than 50% activity after 72 h at 30-40 or 50°C, respectively | Talaromyces amestolkiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
39.2 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
51.2 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
66.1 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
143 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
173 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
183 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
220 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
229 | - |
beechwood xylan | at pH 3.0 and 50°C | Talaromyces amestolkiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Talaromyces amestolkiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 5.5 | about 95% activity at pH 2.2, 100% activity at pH 3.0, about 90% activity at pH 4.0, about 55% activity at pH 5.5, about 42% activity at pH 6.0, about 12% activity at pH 7.0 | Talaromyces amestolkiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.2 | 9 | the enzyme retains more than 80% activity after 72 h at pH 2.2-9.0 | Talaromyces amestolkiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
D-xylose | pH and temperature not specified in the publication | Talaromyces amestolkiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Talaromyces amestolkiae | isoelectric focusing | - |
7.6 |
Organism | Comment | Expression |
---|---|---|
Talaromyces amestolkiae | the expression of the enzyme can be induced by 1% (w/v) Avicel, 1% (w/v) D-xylose, or 1-3% (w/v) beechwood xylan | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
62 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
140 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
200 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
260 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
290 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
380 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
1000 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae |