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Literature summary for 3.2.1.37 extracted from

  • Nieto-Dominguez, M.; de Eugenio, L.I.; Barriuso, J.; Prieto, A.; Fernandez de Toro, B.; Canales-Mayordomo, A.; Martinez, M.J.
    Novel pH-stable glycoside hydrolase family 3 beta-xylosidase from Talaromyces amestolkiae an enzyme displaying regioselective transxylosylation (2015), Appl. Environ. Microbiol., 81, 6380-6392 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Talaromyces amestolkiae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ about 50% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
Cu2+ about 75% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
D-xylose product inhibition Talaromyces amestolkiae
Hg2+ about 10% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
additional information absence of inhibition in the presence of EDTA, dithiothreitol, and 2-mercaptoethanol Talaromyces amestolkiae
Ni2+ about 75% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
Pb2+ about 90% residual activity at 5 mM in citrate buffer Talaromyces amestolkiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
0.2
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
0.2
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
0.22
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
0.22
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
0.48
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
3.6
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
5.8
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ about 120% activity at 5 mM in acetate buffer Talaromyces amestolkiae
Co2+ about 110% activity at 5 mM in acetate buffer Talaromyces amestolkiae
Fe2+ about 125% activity at 5 mM in acetate buffer Talaromyces amestolkiae
Mg2+ about 110% activity at 5 mM in acetate buffer Talaromyces amestolkiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Talaromyces amestolkiae

Organism

Organism UniProt Comment Textmining
Talaromyces amestolkiae A0A0H3W535
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Talaromyces amestolkiae

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography, Mono S column chromatography, and Superose 12 gel filtration Talaromyces amestolkiae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Talaromyces amestolkiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + L-arabinopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + D-xylopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + xylobiose
-
Talaromyces amestolkiae 4-nitrophenol + xylotriose
-
?
4-nitrophenyl beta-D-xylopyranoside + xylotriose
-
Talaromyces amestolkiae 4-nitrophenol + xylotetraose
-
?
beechwood xylan + H2O
-
Talaromyces amestolkiae D-xylose + ?
-
?
additional information no activity with 4-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-fucopyranoside Talaromyces amestolkiae ?
-
?
xylan + H2O
-
Talaromyces amestolkiae ?
-
?
xylobiose + H2O
-
Talaromyces amestolkiae 2 D-xylose
-
?
xylohexaose + H2O
-
Talaromyces amestolkiae 6 D-xylose
-
?
xylopentaose + H2O
-
Talaromyces amestolkiae 5 D-xylose
-
?
xylotetraose + 3 H2O
-
Talaromyces amestolkiae 4 D-xylose
-
?
xylotriose + H2O
-
Talaromyces amestolkiae 3 D-xylose
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 100000, SDS-PAGE Talaromyces amestolkiae
homodimer 2 * 102275, MALDI-TOF mass spectrometry Talaromyces amestolkiae
homodimer 2 * 84374, calculated from amino acid sequence Talaromyces amestolkiae

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
Talaromyces amestolkiae
BxTW1
-
Talaromyces amestolkiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Talaromyces amestolkiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 more than 50% activity between 50 and 70°C Talaromyces amestolkiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme retains more than 70% activity and more than 50% activity after 72 h at 30-40 or 50°C, respectively Talaromyces amestolkiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
39.2
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
51.2
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
66.1
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
143
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae
173
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
183
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
220
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
229
-
beechwood xylan at pH 3.0 and 50°C Talaromyces amestolkiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Talaromyces amestolkiae

pH Range

pH Minimum pH Maximum Comment Organism
2.2 5.5 about 95% activity at pH 2.2, 100% activity at pH 3.0, about 90% activity at pH 4.0, about 55% activity at pH 5.5, about 42% activity at pH 6.0, about 12% activity at pH 7.0 Talaromyces amestolkiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 9 the enzyme retains more than 80% activity after 72 h at pH 2.2-9.0 Talaromyces amestolkiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7
-
D-xylose pH and temperature not specified in the publication Talaromyces amestolkiae

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces amestolkiae isoelectric focusing
-
7.6

Expression

Organism Comment Expression
Talaromyces amestolkiae the expression of the enzyme can be induced by 1% (w/v) Avicel, 1% (w/v) D-xylose, or 1-3% (w/v) beechwood xylan up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae
62
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
140
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
200
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
260
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
290
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
380
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
1000
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae