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Literature summary for 3.2.1.37 extracted from

  • Huang, Y.; Zheng, X.; Pilgaard, B.; Holck, J.; Muschiol, J.; Li, S.; Lange, L.
    Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea (2019), Appl. Microbiol. Biotechnol., 103, 777-791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Pichia pastoris Rhizophlyctis rosea

Inhibitors

Inhibitors Comment Organism Structure
D-xylose this enzyme shows low product inhibition and retained 51% activity in the presence of 100 mM xylose Rhizophlyctis rosea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.34
-
 4-nitrophenyl beta-D-xylopyranoside 25°C, pH 7.0 Rhizophlyctis rosea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 calculated from sequence Rhizophlyctis rosea

Organism

Organism UniProt Comment Textmining
Rhizophlyctis rosea A0A2U8LMQ1
-
-
Rhizophlyctis rosea NBRC 105426 A0A2U8LMQ1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information there is no predicted N-glycosylation site for RrXyl43A Rhizophlyctis rosea

Purification (Commentary)

Purification (Comment) Organism
-
 Rhizophlyctis rosea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Rhizophlyctis rosea 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Rhizophlyctis rosea NBRC 105426 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rhizophlyctis rosea 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rhizophlyctis rosea NBRC 105426 4-nitrophenol + beta-D-xylopyranose
-
 ?

Synonyms

Synonyms Comment Organism
GH43 xylosidase
-
 Rhizophlyctis rosea
RrXyl43A
-
 Rhizophlyctis rosea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Rhizophlyctis rosea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, 72% residual activity Rhizophlyctis rosea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.2
-
 4-nitrophenyl beta-D-xylopyranoside 25°C, pH 7.0 Rhizophlyctis rosea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhizophlyctis rosea

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable Rhizophlyctis rosea