Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-D-fructose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
beta-D-glucose | promotes beta-D-fucosidase activity at 50 mM, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
beta-D-mannose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
beta-D-xylose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb |
Application | Comment | Organism |
---|---|---|
synthesis | synthesis of fucosylsugars using the transglycosylation activity | Bifidobacterium breve clb |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-amino-1-deoxy-D-glucose | - |
Bifidobacterium breve clb | |
1-butanol | inhibition above 50 mM | Bifidobacterium breve clb | |
1-propanol | inhibition above 50 mM | Bifidobacterium breve clb | |
4-O-beta-D-galactopyranosyl-D-glucose | - |
Bifidobacterium breve clb | |
4-O-beta-D-glucopyranosyl-D-glucose | cellobiose | Bifidobacterium breve clb | |
acetone | inhibition at low concentrations | Bifidobacterium breve clb | |
acetonitrile | inhibition at low concentrations | Bifidobacterium breve clb | |
Ag+ | inhibition at 1 mM | Bifidobacterium breve clb | |
beta-D-fructofuranosyl-alpha-D-glucopyranoside | - |
Bifidobacterium breve clb | |
beta-D-fucose | inhibition of beta-D-glucosidase activity, beta-D-fucosidase activity hardly affected | Bifidobacterium breve clb | |
beta-D-glucose | inhibition of beta-D-glucosidase activity, not beta-D-fucosidase activity | Bifidobacterium breve clb | |
Cu2+ | inhibition at 1 mM | Bifidobacterium breve clb | |
ethanol | inhibition above 50 mM | Bifidobacterium breve clb | |
Hg2+ | inhibition at 1 mM | Bifidobacterium breve clb | |
methanol | inhibition above 50 mM | Bifidobacterium breve clb | |
p-chloromercuribenzoate | inhibition at 0.01 mM | Bifidobacterium breve clb |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
p-nitrophenyl-beta-D-fucoside | - |
Bifidobacterium breve clb | |
1.3 | - |
p-nitrophenyl-beta-D-glucoside | - |
Bifidobacterium breve clb |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | 48000 | native gradient PAGE, SDS-PAGE | Bifidobacterium breve clb |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucoside + H2O | Bifidobacterium breve clb | 80% activity compared to beta-D-glucoside | beta-D-fucose + 4-nitrophenol | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | Bifidobacterium breve clb | 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction | beta-D-glucose + 4-nitrophenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium breve clb | - |
increased activity after acclimation with cellobiose | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by chromatography steps | Bifidobacterium breve clb |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bifidobacterium breve clb | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.6 | - |
for beta-D-fucosidase activity | Bifidobacterium breve clb |
67.3 | - |
for beta-D-glucosidase activity | Bifidobacterium breve clb |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | - |
Bifidobacterium breve clb | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucoside + H2O | increased activity after acclimation of bacteria to cellobiose medium | Bifidobacterium breve clb | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | 80% activity compared to beta-D-glucoside | Bifidobacterium breve clb | beta-D-fucose + 4-nitrophenol | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction | Bifidobacterium breve clb | beta-D-glucose + 4-nitrophenol | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose, weak activity after prolonged incubation | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
p-nitrophenyl-beta-D-fucoside + H2O | 80% activity compared to the activity towards beta-D-glucoside | Bifidobacterium breve clb | p-nitrophenol + beta-D-fucose | - |
? | |
p-nitrophenyl-beta-D-fucoside + H2O | contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside | Bifidobacterium breve clb | p-nitrophenol + beta-D-fucose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | low activity | Bifidobacterium breve clb | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-glucoside + H2O | - |
Bifidobacterium breve clb | p-nitrophenol + beta-D-glucose | - |
? | |
p-nitrophenyl-beta-D-xyloside + H2O | low activity | Bifidobacterium breve clb | p-nitrophenol + beta-D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000-48000, SDS-PAGE, native PAGE | Bifidobacterium breve clb |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucosidase/beta-D-fucosidase | - |
Bifidobacterium breve clb |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bifidobacterium breve clb |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to, 60% activity after incubation at 50°C | Bifidobacterium breve clb |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Bifidobacterium breve clb |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | 90% activity between pH 5-7, 70% activity remained at pH 4.5 and pH 8.5 | Bifidobacterium breve clb |