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Literature summary for 3.2.1.39 extracted from

  • Fontaine, T.; Hartland, R.P.; Beauvais, A.; Diaquin, M.; Latge, J.P.
    Purification and characterization of an endo-1,3-beta-glucanase from Aspergillus fumigatus (1997), Eur. J. Biochem., 243, 315-321.
    View publication on PubMed
Search for more literature for 3.2.1.39

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ inhibition at 0.4 mM Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 reduced curdlan value in mg/ml: 1.18 Aspergillus fumigatus
additional information
-
 reduced laminarin value in mg/ml: 0.3 Aspergillus fumigatus
additional information
-
 reduced laminarioctaose value in mg/ml: 0.3 Aspergillus fumigatus
additional information
-
 reduced laminaripentaose value in mg/ml: 0.3 Aspergillus fumigatus
additional information
-
 reduced lamanrihexaose value in mg/ml: 0.2 Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Aspergillus fumigatus 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 SDS-PAGE, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkali soluble 1,3-beta-glucan + H2O Aspergillus fumigatus produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus reducing sugar + ?
-
 ?
cell wall glucan + H2O Aspergillus fumigatus putative role during cell wall morphogenesis reducing sugar + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylated with mannose residues, only O-linked Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 4step chroamtography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O low activity Aspergillus fumigatus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaritetraose Aspergillus fumigatus ?
-
 ?
alkali soluble 1,3-beta-glucan + H2O produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus Aspergillus fumigatus reducing sugar + ?
-
 ?
cell wall glucan + H2O putative role during cell wall morphogenesis Aspergillus fumigatus reducing sugar + ?
-
 ?
curdlan + H2O insoluble reduced 1,3-beta-glucan Aspergillus fumigatus ?
-
 ?
laminarin + H2O soluble reduced 1,3-beta-glucan, release of reducing 1,3-beta-oligosaccharides of variable size, presence of a single 1,6-beta branched glucose every three glucose units of a linear 1,3-beta-glucan chain prevents hydrolysis Aspergillus fumigatus D-glucose + ?
-
 ?
reduced laminarioligosaccharides + H2O velocity of catalytic reaction increases with the number of 1,3-beta-glucosidic bonds, preferentially cleaves between the glucose residues at positions 2 and 3 from the reducing end Aspergillus fumigatus laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE, gel filtration Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable up to, activity lost at 70°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 stable within Aspergillus fumigatus