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Literature summary for 3.2.1.4 extracted from
- Purification and properties of a cellulase from Aspergillus niger (1985), Agric. Biol. Chem., 49, 1257-1265.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 75% inhibition | Aspergillus niger |  |
| Fe2+ | 1 mM, 55% inhibition | Aspergillus niger | |
| Fe3+ | - |
Aspergillus niger | |
| Hg2+ | 1 mM, 67% inhibition | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus niger |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | contains no carbohydrate | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 116.8 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Aspergillus niger | additional information | - |
? | |
| cellohexaose + H2O | - |
Aspergillus niger | additional information | cellotriose + glucose + cellobiose + cellotetraose | ? | |
| cellopentaose + H2O | - |
Aspergillus niger | additional information | - |
? | |
| cellotetraose + H2O | - |
Aspergillus niger | 2 cellobiose | + glucose + cellotriose | ? | |
| cellotriose + H2O | - |
Aspergillus niger | D-glucose + cellobiose | + glucose | ? | |
| additional information | no activity with p-nitrophenyl-beta-D-glucoside | Aspergillus niger | ? | - |
? | |
| additional information | no activity with cellobiose | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 65% of maximal activity, 70°C: about 40% of maximal activity | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, completely stable below | Aspergillus niger |
| 70 | - |
10 min, 50% loss of activity | Aspergillus niger |
| 80 | - |
10 min, complete inactivation | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 4°C, 24 h, stable | Aspergillus niger |
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