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Literature summary for 3.2.1.4 extracted from

  • Louime, C.; Abazinge, M.; Johnson, E.; Latinwo, L.; Ikediobi, C.; Clark, A.M.
    Molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria Cytophaga hutchinsonii (2007), Appl. Biochem. Biotechnol., 141, 127-138.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli with a His-Tag based expression vector Cytophaga hutchinsonii

Organism

Organism UniProt Comment Textmining
Cytophaga hutchinsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cytophaga hutchinsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O Cel9A is a nonprocessive enzyme with endo-cellulase activities Cytophaga hutchinsonii ?
-
 ?
additional information Cel9A does not cleave xylan Cytophaga hutchinsonii ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel9A
-
 Cytophaga hutchinsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cytophaga hutchinsonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 stable Cytophaga hutchinsonii
60
-
 30% loss of activity overnight Cytophaga hutchinsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Cytophaga hutchinsonii

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 40% of maximal activity, pH 9.0: about 55% of maximal activity Cytophaga hutchinsonii

pI Value

Organism Comment pI Value Maximum pI Value
Cytophaga hutchinsonii calculated from sequence
-
 6.5