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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull (2008), Biores. Technol., 99, 378-386.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus amyloliquefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | 5 mM, 35% loss of activity | Bacillus amyloliquefaciens |  |
| EDTA | 5 mM, 75% loss of activity | Bacillus amyloliquefaciens | |
| HgCl2 | 5 mM, 76% loss of activity | Bacillus amyloliquefaciens | |
| KCl | 5 mM, 23% loss of activity | Bacillus amyloliquefaciens | |
| MnCl2 | 5 mM, 67% loss of activity | Bacillus amyloliquefaciens | |
| N-bromosuccinimide | 5 mM, 56% loss of activity | Bacillus amyloliquefaciens | |
| NiCl2 | 5 mM, 40% loss of activity | Bacillus amyloliquefaciens | |
| PbCl2 | 5 mM, 37% loss of activity | Bacillus amyloliquefaciens | |
| SrCl2 | 5 mM, 36% loss of activity | Bacillus amyloliquefaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 5 mM, activation to 109% of control | Bacillus amyloliquefaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Bacillus amyloliquefaciens |
| 55118 | - |
x * 55118, calculated from sequence | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus amyloliquefaciens DL-3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus amyloliquefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | - |
Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | 14% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
| avicel + H2O | 14% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
| beta-glucan + H2O | 24.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
| beta-glucan + H2O | 24.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
| cellobiose + H2O | 8.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
| additional information | no activity with p-nitrophenyl-beta-D-glucopyranoside | Bacillus amyloliquefaciens | ? | - |
? | |
| additional information | no activity with p-nitrophenyl-beta-D-glucopyranoside | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
| xylan + H2O | 22.5% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
| xylan + H2O | 22.5% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Bacillus amyloliquefaciens |
| ? | x * 55118, calculated from sequence | Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 8.0, substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | 20°C: 65.9% of maximal activity, 80°C: 72.3% of maximal activity, substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | pH 8.0, 20 h, about 60%% loss of activity | Bacillus amyloliquefaciens |
| 40 | - |
pH 8.0, 20 h, about 40% loss of activity | Bacillus amyloliquefaciens |
| 50 | - |
pH 8.0, 20 h, about 10% loss of activity | Bacillus amyloliquefaciens |
| 60 | - |
pH 8.0, 20 h, about 15% loss of activity | Bacillus amyloliquefaciens |
| 70 | - |
pH 8.0, 20 h, about 20% loss of activity | Bacillus amyloliquefaciens |
| 80 | - |
pH 8.0, 20 h, about 40% loss of activity | Bacillus amyloliquefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | pH 3: about 55% of maximal activity, pH 11: about 45% of maximal activity | Bacillus amyloliquefaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 50°C, 20 h, stable | Bacillus amyloliquefaciens |
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