Cloned (Comment) | Organism |
---|---|
cloned Xf818, into expression vectors pET20b and pET28b, expression in Escherichia coli | Xylella fastidiosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Xylella fastidiosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xylella fastidiosa | Q9PF60 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant form of Xf818 containing a His6 tag | Xylella fastidiosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acid swollen cellulose + H2O | endoglucanase with exohydrolytic activity | Xylella fastidiosa | cellobiose + cellotriose | - |
? | |
avicel + H2O | cellooligosaccharides production from Avicel is detected only when 0.00185 mkat of Xf818his are employed, and not when 16.67 nkat are used. Endoglucanase with exohydrolytic activity | Xylella fastidiosa | cellobiose + cellotriose | - |
? | |
carboxymethyl cellulose + H2O | endoglucanase with exohydrolytic activity | Xylella fastidiosa | ? | - |
? | |
cellopentaose + H2O | - |
Xylella fastidiosa | ? | - |
? | |
cellotetraose + H2O | - |
Xylella fastidiosa | ? | - |
? | |
additional information | Xf818 carries out transglycosylation. The enzyme is unable to hydrolyse alpha-cellulose and laminarin (a beta-1,3 linked glucan) | Xylella fastidiosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Xylella fastidiosa |
Synonyms | Comment | Organism |
---|---|---|
cellulase Xf818 | - |
Xylella fastidiosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
wild-type cellulase Xf818 and recombinant His-tagged cellulase Xf818his | Xylella fastidiosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 45% of maximal activity, 70°C: about 60% of maximal activity, recombinant His-tagged cellulase Xf818his | Xylella fastidiosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
wild-type cellulase Xf818 | Xylella fastidiosa |
5.2 | - |
recombinant His-tagged cellulase Xf818his | Xylella fastidiosa |