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Literature summary for 3.2.1.4 extracted from

  • Wulff, N.A.; Carrer, H.; Pascholati, S.F.
    Expression and purification of cellulase Xf818 from Xylella fastidiosa in Escherichia coli (2006), Curr. Microbiol., 53, 198-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned Xf818, into expression vectors pET20b and pET28b, expression in Escherichia coli Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Xylella fastidiosa

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa Q9PF60
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant form of Xf818 containing a His6 tag Xylella fastidiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid swollen cellulose + H2O endoglucanase with exohydrolytic activity Xylella fastidiosa cellobiose + cellotriose
-
?
avicel + H2O cellooligosaccharides production from Avicel is detected only when 0.00185 mkat of Xf818his are employed, and not when 16.67 nkat are used. Endoglucanase with exohydrolytic activity Xylella fastidiosa cellobiose + cellotriose
-
?
carboxymethyl cellulose + H2O endoglucanase with exohydrolytic activity Xylella fastidiosa ?
-
?
cellopentaose + H2O
-
Xylella fastidiosa ?
-
?
cellotetraose + H2O
-
Xylella fastidiosa ?
-
?
additional information Xf818 carries out transglycosylation. The enzyme is unable to hydrolyse alpha-cellulose and laminarin (a beta-1,3 linked glucan) Xylella fastidiosa ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Xylella fastidiosa

Synonyms

Synonyms Comment Organism
cellulase Xf818
-
Xylella fastidiosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
wild-type cellulase Xf818 and recombinant His-tagged cellulase Xf818his Xylella fastidiosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: about 45% of maximal activity, 70°C: about 60% of maximal activity, recombinant His-tagged cellulase Xf818his Xylella fastidiosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
wild-type cellulase Xf818 Xylella fastidiosa
5.2
-
recombinant His-tagged cellulase Xf818his Xylella fastidiosa