Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
I62T/L79I/A93T/S308P/I370V/L374P/M416V/F472I/I484V/W494R | S40 mutant, DNA shuffling. Higher hydrolytic activities than the wild-type enzyme | Bacillus subtilis |
K120E/D272H/S283G/S308P/L374P | M1-23 mutant, random mutation. Higher hydrolytic activities than the wild-type enzyme | Bacillus subtilis |
K120E/S283G/S308P/L374P | M1 mutant, random mutation. Higher hydrolytic activities than the wild-type enzyme, shows 1.25fold increase in activity | Bacillus subtilis |
additional information | variants (M44-11, S75 and S78) show 2.03fold to 2.68fold increased activities toward sodium carboxymethyl cellulose | Bacillus subtilis |
N39D/K120E/N175H/V255A/S308P/L386S/K398R | S75 mutant, DNA shuffling. Higher hydrolytic activities than the wild-type enzyme | Bacillus subtilis |
T32I/N39D/K120E/S248G/S283G/S308P/R314G/I370N/L374P/N403D/N451D/S467N | S78 mutant, DNA shuffling. Higher hydrolytic activities than the wild-type enzyme | Bacillus subtilis |
V74A/K120E/D272G/K337E/S355P/D459G/K479E/K482E/K491N | M44 mutant, random mutation. Higher hydrolytic activities than the wild-type enzyme, shows 1.56fold increase in activity | Bacillus subtilis |
V74A/K120E/D272G/K337E/S355P/T449I/D459G/K479E/K482E/D488N/K491N | M44-11 mutant, random mutation. Higher hydrolytic activities than the wild-type enzyme | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52170 | - |
- |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
BME-15 | - |
Bacillus subtilis BME-15 | - |
BME-15 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.91 | - |
Cel5A, carboxymethyl cellulose as substrate | Bacillus subtilis |
3.88 | - |
M44-11 mutant, carboxymethyl cellulose as substrate | Bacillus subtilis |
4.84 | - |
S75 mutant, carboxymethyl cellulose as substrate | Bacillus subtilis |
4.88 | - |
S78 mutant, carboxymethyl cellulose as substrate | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley glucan + H2O | - |
Bacillus subtilis | ? | - |
? | |
barley glucan + H2O | - |
Bacillus subtilis BME-15 | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus subtilis BME-15 | ? | - |
? | |
cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
cellulose + H2O | - |
Bacillus subtilis BME-15 | ? | - |
? | |
sodium carboxymethyl cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
sodium carboxymethyl cellulose + H2O | - |
Bacillus subtilis BME-15 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel5A | - |
Bacillus subtilis |
Cellulase | - |
Bacillus subtilis |
endo-beta-1,4-glucanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
S75 mutant | Bacillus subtilis |
50 | - |
Cel5A wild-type and M44-11 mutant | Bacillus subtilis |
60 | - |
S78 mutant | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
M44-11 mutant shows higher stability than others and retains more than 50% of its activity after incubation at 80°C for 1 h | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
for mutants M44-11, S75, and S78 | Bacillus subtilis |