Application | Comment | Organism |
---|---|---|
industry | due to its acidophilic (pH 3.5) and high temperature stability (up to 90°C), CSCMCase may be useful for industrial application such as animal feed industry and clarification of fruit juices | Cryptococcus sp. S-2 |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Cryptococcus sp. S-2 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Cryptococcus sp. S-2 |
34070 | - |
calculated | Cryptococcus sp. S-2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus sp. S-2 | A4UU22 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity from the supernatant by ultrafiltration, DEAE-5PW anion exchange column and TSK-gel G3000SW gel filtration | Cryptococcus sp. S-2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
CM cellulose | Cryptococcus sp. S-2 |
4.93 | - |
carboxymethyl cellulose sodium salt | Cryptococcus sp. S-2 |
248.7 | - |
recombinant CSCMCase | Cryptococcus sp. S-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? | |
cellobiose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? | |
CM cellulose + H2O | - |
Cryptococcus sp. S-2 | ? | - |
? | |
additional information | carboxymethyl cellulose sodium salt is a better substrate than insoluble cellulose substrate such as Avicel (0 U/mg), SIGMACELL cellulose (0 U/mg), and CM cellulose | Cryptococcus sp. S-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Cryptococcus sp. S-2 |
Synonyms | Comment | Organism |
---|---|---|
Carboxymethyl cellulase | - |
Cryptococcus sp. S-2 |
CSCMCase | - |
Cryptococcus sp. S-2 |
thermostable carboxymethyl cellulase | - |
Cryptococcus sp. S-2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Cryptococcus sp. S-2 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
enzyme retains approximately 50% of its maximum activity after incubating at 90°C for 1 h | Cryptococcus sp. S-2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
maximum carboxymethyl cellulase activity at | Cryptococcus sp. S-2 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | - |
Cryptococcus sp. S-2 |