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Literature summary for 3.2.1.4 extracted from

  • Saleem, M.; Akhtar, M.S.; Yasmin, R.; Zahid, M.; Malik, N.N.; Afzal, M.; Rajoka, M.I.
    Production, purification and characterization of beta-1,4-endoglucanase from a novel bacterial strain CTP-09 of a Bacillus sp (2008), Protein Pept. Lett., 15, 402-410.
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 40% enhanced activity Bacillus sp. (in: Bacteria)
Mg2+ 32% enhanced activity Bacillus sp. (in: Bacteria)

Application

Application Comment Organism
industry enzyme possesses high power of defibrillation of textile and laundry. Polypeptide can be exploited for mass production and application in local industries Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 78% inhibition Bacillus sp. (in: Bacteria)
EDTA 78% inhibition Bacillus sp. (in: Bacteria)
Fe2+ 25% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 78% inhibition Bacillus sp. (in: Bacteria)
additional information is minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme Bacillus sp. (in: Bacteria)
SDS 78% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 52% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values: 4.8 mg/ml for carboxymethyl cellulose at 50 and 60°C, 4.5 mg/ml for carboxymethyl cellulose at 70°C, 4.2 mg/ml for carboxymethyl cellulose at 80°C, and 18.4 mg/ml for amorphous cellulose Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information effect of metal ions tested: Ca2+, Mg2+, Hg2+, Fe2+, Cu2+, Zn2+, SDS, dithiothreitol and metal chelator such as EDTA Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)
48000
-
 gel filtration, pH 8.5 Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 CTP-09
-
Bacillus sp. (in: Bacteria) CTP-09
-
 CTP-09
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bacillus sp. (in: Bacteria)

Purification (Commentary)

Purification (Comment) Organism
combination of ammonium sulfate precipitation, gel filtration, and ion exchange chromatographic techniques Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
450
-
 after ion-exchange chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2% Bacillus sp. (in: Bacteria) ?
-
 ?
avicel + H2O microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2% Bacillus sp. (in: Bacteria) CTP-09 ?
-
 ?
carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
carboxymethylcellulose + H2O
-
 Bacillus sp. (in: Bacteria) CTP-09 ?
-
 ?
cellulose + H2O amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%) Bacillus sp. (in: Bacteria) ?
-
 ?
cellulose + H2O amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%) Bacillus sp. (in: Bacteria) CTP-09 ?
-
 ?
additional information investigation of substrate specificity Bacillus sp. (in: Bacteria) ?
-
 ?
additional information starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9% Bacillus sp. (in: Bacteria) ?
-
 ?
additional information investigation of substrate specificity Bacillus sp. (in: Bacteria) CTP-09 ?
-
 ?
additional information starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9% Bacillus sp. (in: Bacteria) CTP-09 ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
beta-1,4-endoglucanase
-
 Bacillus sp. (in: Bacteria)
CMCase
-
 Bacillus sp. (in: Bacteria)
endo-glucanase
-
 Bacillus sp. (in: Bacteria)
endoglucanase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 high level of activity is produced when organism is grown at 55°C Bacillus sp. (in: Bacteria)
60
-
 assay at Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 stable up to Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 optimal pH at 80°C Bacillus sp. (in: Bacteria)
7.5
-
 optimal pH at 60°C and assay at Bacillus sp. (in: Bacteria)
7.8
-
 optimal pH at 50°C Bacillus sp. (in: Bacteria)
8.1
-
 optimal pH at 70°C Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Bacillus sp. (in: Bacteria)