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Literature summary for 3.2.1.4 extracted from

  • Bao, L.; Huang, Q.; Chang, L.; Zhou, J.; Lu, H.
    Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome (2011), J. Mol. Catal. B, 73, 104-110.
No PubMed abstract available
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged Rucel5B in Escherichia coli strain BL21(DE3) uncultured rumen bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 uncultured rumen bacterium
Cu2+
-
 uncultured rumen bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of Rucel5B with different substrates, overview uncultured rumen bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates uncultured rumen bacterium
Mg2+ activates uncultured rumen bacterium
additional information no effect by Zn2+ on Rucel5B activity uncultured rumen bacterium
Ni2+ activates uncultured rumen bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38400
-
 x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium
40000
-
 x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium

Organism

Organism UniProt Comment Textmining
uncultured rumen bacterium G3BJX5 from rumen of Bos mutus, Rucel5B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Rucel5B from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured rumen bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
 Rucel5B, pH 6.5, 60°C, substrate carboxymethyl cellulose uncultured rumen bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 uncultured rumen bacterium ?
-
 ?
additional information Rucel5B is active towards carboxymethyl cellulose, barley glucan, lichenan, pNPC, PASC, avicel and Whatman NO.1 filter paper, but not active towards laminarin, beta-1,6-glucan and birchwood xylan uncultured rumen bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, recombinant His-tagged Rucel5B, SDS-PAGE, x * 38400, about, Rucel5B, sequence calculation uncultured rumen bacterium

Synonyms

Synonyms Comment Organism
beta-1,4-endoglucanase
-
 uncultured rumen bacterium
Rucel5B
-
 uncultured rumen bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 uncultured rumen bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 activity range of Rucel5B, profile, overview uncultured rumen bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
163
-
 carboxymethyl cellulose Rucel5B, pH 6.5, 60°C uncultured rumen bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 uncultured rumen bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range of Rucel5B, profile, overview uncultured rumen bacterium