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Literature summary for 3.2.1.4 extracted from

  • Huang, Y.; Krauss, G.; Cottaz, S.; Driguez, H.; Lipps, G.
    A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus (2005), Biochem. J., 385, 581-588.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate pH 1.8, 80°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Saccharolobus solfataricus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Saccharolobus solfataricus
37508
-
x * 37508, calculated from sequence Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Saccharolobus solfataricus the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source cellobiose + ?
-
?
cellulose + H2O Saccharolobus solfataricus P2 the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VS7
-
-
Saccharolobus solfataricus Q97X08
-
-
Saccharolobus solfataricus P2 Q97VS7
-
-
Saccharolobus solfataricus P2 Q97X08
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 1.8, 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Saccharolobus solfataricus cellobiose + ?
-
?
carboxymethylcellulose + H2O
-
Saccharolobus solfataricus P2 cellobiose + ?
-
?
cellopentaose + H2O
-
Saccharolobus solfataricus cellobiose + cellotriose
-
?
cellopentaose + H2O
-
Saccharolobus solfataricus P2 cellobiose + cellotriose
-
?
cellotetraose + H2O minimal substrate length, cellotriose and cellobiose are not hydrolyzed Saccharolobus solfataricus 2 cellobiose
-
?
cellotetraose + H2O minimal substrate length, cellotriose and cellobiose are not hydrolyzed Saccharolobus solfataricus P2 2 cellobiose
-
?
cellulose + H2O the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source Saccharolobus solfataricus cellobiose + ?
-
?
cellulose + H2O the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source Saccharolobus solfataricus P2 cellobiose + ?
-
?
additional information the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values Saccharolobus solfataricus ?
-
?
additional information the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values Saccharolobus solfataricus P2 ?
-
?
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O
-
Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Saccharolobus solfataricus
? x * 37508, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO1949 locus name Saccharolobus solfataricus
SSO2534
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 70°C: about 60% of maximal activity, 95°C: about 40% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life: 8 h at pH 1.8, 160 min at pH 1. At neutral pH, the thermal inactivation rate is nearly two orders of magnitude higher than at pH 1.8 Saccharolobus solfataricus
95
-
pH 1.8, rapid inactivation Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
1.2 2.5 pH 1.2: about 50% of maximal activity, pH 2-5: about 70% of maximal activity Saccharolobus solfataricus