Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Chahed, H.; Ezzine, A.; Mlouka, A.B.; Hardouin, J.; Jouenne, T.; Marzouki, M.N.
    Biochemical characterization, molecular cloning, and structural modeling of an interesting beta-1,4-glucanase from Sclerotinia sclerotiorum (2014), Mol. Biotechnol., 56, 340-350.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Sclerotinia sclerotiorum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Sclerotinia sclerotiorum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, SDS-PAGE Sclerotinia sclerotiorum
33297
-
x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum
34000
-
x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum A7E948
-
-
Sclerotinia sclerotiorum P21833
-
-
Sclerotinia sclerotiorum P21834
-
-
Sclerotinia sclerotiorum 1980 A7E948
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum ?
-
?
carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum 1980 ?
-
?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Sclerotinia sclerotiorum
? x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum
More the enzyme contains two domains, a core domain having a high similarity with endoglucanases family 5 and a cellulose-binding domain having similarities with those of exo-type cellulases of family 1, linked together by a serine-threonine-rich region, peptide mapping, mass spectrometric analysis Sclerotinia sclerotiorum

Synonyms

Synonyms Comment Organism
CMCase
-
Sclerotinia sclerotiorum
endo-beta-1,4-glucanase
-
Sclerotinia sclerotiorum
endo-beta-D-1,4-glucanohydrolase
-
Sclerotinia sclerotiorum
ENDO1
-
Sclerotinia sclerotiorum
ENDO2
-
Sclerotinia sclerotiorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Sclerotinia sclerotiorum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range, profile overview Sclerotinia sclerotiorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Sclerotinia sclerotiorum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 activity range, profile overview Sclerotinia sclerotiorum

pI Value

Organism Comment pI Value Maximum pI Value
Sclerotinia sclerotiorum sequence calculation
-
6.43

Expression

Organism Comment Expression
Sclerotinia sclerotiorum extracellular endo-beta-1,4-glucanase isozyme Endo1 is induced by carboxymethyl cellulose up
Sclerotinia sclerotiorum extracellular endo-beta-1,4-glucanase isozyme Endo2 is induced by carboxymethyl cellulose up

General Information

General Information Comment Organism
evolution isozyme Endo1 belongs to the glycosyl hydrolase family 45, GH45 Sclerotinia sclerotiorum
additional information protein structure homology molecular modeling and three-dimensional structure comparison, overview Sclerotinia sclerotiorum