Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Sclerotinia sclerotiorum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Sclerotinia sclerotiorum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Sclerotinia sclerotiorum |
33297 | - |
x * 33297, sequence calculation, x * 34000, SDS-PAGE | Sclerotinia sclerotiorum |
34000 | - |
x * 33297, sequence calculation, x * 34000, SDS-PAGE | Sclerotinia sclerotiorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia sclerotiorum | A7E948 | - |
- |
Sclerotinia sclerotiorum | P21833 | - |
- |
Sclerotinia sclerotiorum | P21834 | - |
- |
Sclerotinia sclerotiorum 1980 | A7E948 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | release of reducing sugar, degradation | Sclerotinia sclerotiorum | ? | - |
? | |
carboxymethyl cellulose + H2O | release of reducing sugar, degradation | Sclerotinia sclerotiorum 1980 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Sclerotinia sclerotiorum |
? | x * 33297, sequence calculation, x * 34000, SDS-PAGE | Sclerotinia sclerotiorum |
More | the enzyme contains two domains, a core domain having a high similarity with endoglucanases family 5 and a cellulose-binding domain having similarities with those of exo-type cellulases of family 1, linked together by a serine-threonine-rich region, peptide mapping, mass spectrometric analysis | Sclerotinia sclerotiorum |
Synonyms | Comment | Organism |
---|---|---|
CMCase | - |
Sclerotinia sclerotiorum |
endo-beta-1,4-glucanase | - |
Sclerotinia sclerotiorum |
endo-beta-D-1,4-glucanohydrolase | - |
Sclerotinia sclerotiorum |
ENDO1 | - |
Sclerotinia sclerotiorum |
ENDO2 | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sclerotinia sclerotiorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | activity range, profile overview | Sclerotinia sclerotiorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Sclerotinia sclerotiorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | activity range, profile overview | Sclerotinia sclerotiorum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sclerotinia sclerotiorum | sequence calculation | - |
6.43 |
Organism | Comment | Expression |
---|---|---|
Sclerotinia sclerotiorum | extracellular endo-beta-1,4-glucanase isozyme Endo1 is induced by carboxymethyl cellulose | up |
Sclerotinia sclerotiorum | extracellular endo-beta-1,4-glucanase isozyme Endo2 is induced by carboxymethyl cellulose | up |
General Information | Comment | Organism |
---|---|---|
evolution | isozyme Endo1 belongs to the glycosyl hydrolase family 45, GH45 | Sclerotinia sclerotiorum |
additional information | protein structure homology molecular modeling and three-dimensional structure comparison, overview | Sclerotinia sclerotiorum |