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Literature summary for 3.2.1.4 extracted from

  • Dipasquale, L.; Romano, I.; Picariello, G.; Calandrelli, V.; Lama, L.
    Characterization of a native cellulase activity from an anaerobic thermophilic hydrogen-producing bacterium Thermosipho sp. strain 3 (2014), Ann. Microbiol., 64, 1493-1503.
    View publication on PubMed
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 76% residual activity Thermosipho sp. LD-2008
Cu2+ 5 mM, 12% residual activity Thermosipho sp. LD-2008
Hg2+ 5 mM, no residual activity Thermosipho sp. LD-2008
Ni2+ 5 mM, no residual activity Thermosipho sp. LD-2008
Pb2+ 5 mM, 9% residual activity Thermosipho sp. LD-2008
Zn2+ 5 mM, no residual activity Thermosipho sp. LD-2008

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 5 mM, 1.5fold stimulation Thermosipho sp. LD-2008
Mn2+ 5 mM, 2.4fold stimulation Thermosipho sp. LD-2008

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Thermosipho sp. LD-2008

Organism

Organism UniProt Comment Textmining
Thermosipho sp. LD-2008
-
 DSM 27729
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermosipho sp. LD-2008

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
 pH 5.5, 80°C, substrate carboxymethyl cellulose Thermosipho sp. LD-2008

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O 0.16% of the activity with carboxymethyl cellulose Thermosipho sp. LD-2008 4-nitrophenol + cellobiose
-
 ?
beta-glucan + H2O 400% of the activity with carboxymethyl cellulose Thermosipho sp. LD-2008 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermosipho sp. LD-2008 ?
-
 ?
additional information no substrates: xylan, avicel, alpha-cellulose, filter paper, laminarin, curdlan Thermosipho sp. LD-2008 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Thermosipho sp. LD-2008

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermosipho sp. LD-2008

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 48 h, 50% residual activity Thermosipho sp. LD-2008
70
-
 4 h, 50% residual activity Thermosipho sp. LD-2008

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermosipho sp. LD-2008