Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.5%, 126% of initial activity | Alicyclobacillus vulcanalis | |
Tween 20 | 0.1%, 124% of initial activity | Alicyclobacillus vulcanalis |
Application | Comment | Organism |
---|---|---|
degradation | the purified enzyme decreases the viscosity of carboxymethyl cellulose when assessed at 70-85°C and is capable of releasing reducing sugars from acid-pretreated straw at 70 and 75°C | Alicyclobacillus vulcanalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Alicyclobacillus vulcanalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM, 86% residual activity | Alicyclobacillus vulcanalis | |
additional information | not inhibitory: EDTA, Ca2+ | Alicyclobacillus vulcanalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98466 | - |
x * 98466, calculated | Alicyclobacillus vulcanalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus vulcanalis | A0A0F7TAS1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Alicyclobacillus vulcanalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | 92% of the activity on carboxymethyl cellulose | Alicyclobacillus vulcanalis | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Alicyclobacillus vulcanalis | ? | production of a mixture of cellobiose, cellotriose, cellotetraose and cellopentaose | ? | |
cellopentaose + H2O | - |
Alicyclobacillus vulcanalis | cellobiose + cellotriose | - |
? | |
cellotetraose + H2O | - |
Alicyclobacillus vulcanalis | 2 cellobiose | - |
? | |
lichenan + H2O | 94% of the activity on carboxymethyl cellulose | Alicyclobacillus vulcanalis | ? | - |
? | |
additional information | no substrae: cellobiose | Alicyclobacillus vulcanalis | ? | - |
? | |
xylan + H2O | beechwood xylan, 34% , and birchwood xylan, 31% of the activity on carboxymethyl cellulose, respectively | Alicyclobacillus vulcanalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98466, calculated | Alicyclobacillus vulcanalis |
Synonyms | Comment | Organism |
---|---|---|
celVA | - |
Alicyclobacillus vulcanalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Alicyclobacillus vulcanalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
72 h, presence of 0.1% Tween 20, no loss of activity | Alicyclobacillus vulcanalis |
75 | - |
72 h, presence of 0.1% Tween 20, 75% of initial activity | Alicyclobacillus vulcanalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.6 | 4.5 | - |
Alicyclobacillus vulcanalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alicyclobacillus vulcanalis | calculated | - |
4.3 |