Literature summary for 3.2.1.4 extracted from

Xu, H.; Han, D.; Xu, Z.
Expression of heterologous cellulases in Thermotoga sp. strain RQ2 (2015), Biomed Res. Int., 2015, 304523.

Search for more literature for 3.2.1.4

Application

Application	Comment	Organism
synthesis	expression of enzyme in Escherichia coli and Thermotoga sp. after fusion to the signal peptides of TM1840 (amyA) or TM0070 (xynB). Expressed in Escherichia coli and Thermotoga sp. renders the hosts with increased endo- and exoglucanase activities. In Escherichia coli, the recombinant enzymes are mainly bound to the bacterial cells, whereas in Thermotoga sp., about half of the enzyme activities are observed in the culture supernatants. However, the cellulase activities are lost in Thermotoga sp. after three consecutive transfers	Caldicellulosiruptor saccharolyticus
synthesis	expression of enzyme in Escherichia coli and Thermotoga sp. after fusion to the signal peptides of TM1840 (amyA) or TM0070 (xynB). Expressed in Escherichia coli and Thermotoga sp. renders the hosts with increased endoglucanase activities. In Escherichia coli, the recombinant enzymes are mainly bound to the bacterial cells, whereas in Thermotoga sp., about half of the enzyme activities are observed in the culture supernatants. However, the cellulase activities are lost in Thermotoga sp. after three consecutive transfers	Caldicellulosiruptor saccharolyticus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Thermotoga sp. strain RQ2 and Escherichia coli	Caldicellulosiruptor saccharolyticus

Organism

Organism	UniProt	Comment	Textmining
Caldicellulosiruptor saccharolyticus	P10474	bifunctional endo- and exoglucanase	-
Caldicellulosiruptor saccharolyticus	P22534	-	-

Synonyms

Synonyms	Comment	Organism
CelA	-	Caldicellulosiruptor saccharolyticus
CelB	-	Caldicellulosiruptor saccharolyticus
Csac_1076	-	Caldicellulosiruptor saccharolyticus
Csac_1078	-	Caldicellulosiruptor saccharolyticus

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Release 2023.1 (January 2023)