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Literature summary for 3.2.1.4 extracted from
- Site-directed mutagenesis of a hyperthermophilic endoglucanase Cel12B from Thermotoga maritima based on rational design (2015), PLoS ONE, 10, e0133824.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E225H/K207G | mutant based on homology modeling and rational design, display significantly improved activity and thermostability | Thermotoga maritima |
| E225H/K207G/D37V | mutant based on homology modeling and rational design, display significantly improved activity and thermostability | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.97 | - |
carboxymethyl cellulose | mutant E225H-K207G-D37V, pH 6.0, 85°C | Thermotoga maritima | |
| 2.97 | - |
carboxymethyl cellulose | mutant E225H-K207G, pH 6.0, 85°C | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | G4FFR3 | - |
- |
| Thermotoga maritima DSM 3109 | G4FFR3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Thermotoga maritima | ? | - |
? |  |
| carboxymethyl cellulose + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TM_1525 | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
wild-type | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
8 h, 80% residual activity, mutant E225H-K207G | Thermotoga maritima |
| 80 | - |
8 h, 90% residual activity, mutant E225H-K207G-D37V | Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
wild-type | Thermotoga maritima |
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Release 2023.1 (January 2023)
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