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Literature summary for 3.2.1.4 extracted from

  • Meleiro, L.; Carli, S.; Fonseca-Maldonado, R.; da Silva Torricillas, M.; Zimbardi, A.; Ward, R.; Jorge, J.; Furriel, R.
    Overexpression of a cellobiose-glucose-halotolerant endoglucanase from Scytalidium thermophilum (2017), Appl. Biochem. Biotechnol., 185, 316-333.
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, 1.8fold stimulation Mycothermus thermophilus
cysteine 10 mM, 1.6fold stimulation Mycothermus thermophilus
dithiothreitol 10 mM, 1.8fold stimulation Mycothermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Mycothermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 76% residual activity Mycothermus thermophilus
Hg2+ 1 mM, no residual activity Mycothermus thermophilus
additional information enzyme retains 84.1 and 71.4% of the control activity in the presence of 0.5 and 2.0 mol/l NaCl, respectively Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km value in absence of Mn2+ 10.5 mg/ml, in presence of Mn2+ 7.8 mg/ml, pH 5.5, 60°C Mycothermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese 5 mM, 1.8fold stimulation Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 53% carbohydrate content, molecular mass of the Endo H-treated enzyme is approximately 42 kDa Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.89
-
 substrate filter paper, pH 5.5, 60°C Mycothermus thermophilus
192
-
 substrate medium viscosity carboxymethyl cellulose, pH 5.5, 60°C Mycothermus thermophilus
246
-
 substrate low viscosity carboxymethyl cellulose, pH 5.5, 60°C Mycothermus thermophilus
248
-
 substrate barley beta-glucan, pH 5.5, 60°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Mycothermus thermophilus ?
-
 ?
carboxymethyl cellulose + H2O medium and low viscosity Mycothermus thermophilus ?
-
 ?
filter paper + H2O
-
 Mycothermus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE, 1 * 42000, SDS-PAGE of deglycosylated protein Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life 100 min Mycothermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
475
-
 carboxymethyl cellulose absence of Mn2+, pH 5.5, 60°C Mycothermus thermophilus
677
-
 carboxymethyl cellulose presence of Mn2+, pH 5.5, 60°C Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Mycothermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8
-
 Mycothermus thermophilus