Any feedback?
Literature summary for 3.2.1.4 extracted from
- Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium (2016), J. Biosci. Bioeng., 121, 154-159 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | addition of recombinant Eg5A to cellobiase (cellobiohydrolase and beta-glucosidase) results in a 53% increase in saccharification of NaOH-pretreated barley straw, whereas the glucose release is 47% higher than with cellobiase treatment alone | Phanerodontia chrysosporium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Phanerodontia chrysosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | amino acids, E209 and E319, act as proton donor and nucleophile in the catalytic domain | Phanerodontia chrysosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
carboxymethyl cellulose | pH 5.0, 50°C | Phanerodontia chrysosporium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 177% of initial activity in presence of Mn2+ | Phanerodontia chrysosporium |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q66NB7 | endoglucanase family 5 protein | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | sequence contains a carbohydrate binding module family 1 at the C-terminus, and one potential N-glycosylation site (Asn-Thr-Ser) is found | Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 622 | - |
substrate glucomannan, pH 5.0, 50°C | Phanerodontia chrysosporium |
| 694 | - |
substrate carboxymethyl cellulose, pH 5.0, 50°C | Phanerodontia chrysosporium |
| 1444 | - |
substrate beta-glucan, pH 5.0, 50°C | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Phanerodontia chrysosporium | cellotriose + ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
| glucomannan + H2O | - |
Phanerodontia chrysosporium | ? | - |
? | |
| additional information | very poor substrates: laminarin, xyloglucan, no substrates: birchwood xylan, arabinan, arabinoxylan, mannan, 4-nitrophenyl beta-D-glucopyranoside | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE and calculated | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel5A | - |
Phanerodontia chrysosporium |
| Eg5a | - |
Phanerodontia chrysosporium |
| manganese dependent endoglucanase | - |
Phanerodontia chrysosporium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
50% of maximum activity | Phanerodontia chrysosporium |
| 45 | 70 | - |
Phanerodontia chrysosporium |
| 80 | - |
50% of maximum activity | Phanerodontia chrysosporium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 12 h | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Phanerodontia chrysosporium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Phanerodontia chrysosporium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
