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Literature summary for 3.2.1.4 extracted from

  • Loc, N.; Ngoc, L.; Quang, H.; Huy, N.; Luong, N.
    Cloning and expression of two genes coding endo-beta-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris (2016), Chem. Papers, 70, 284-293 .
No PubMed abstract available
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Trichoderma asperellum

Organism

Organism UniProt Comment Textmining
Trichoderma asperellum X5DQW0 isoform Glu1
-
Trichoderma asperellum X5DTB0 isoform Glu2
-
Trichoderma asperellum PQ34 X5DQW0 isoform Glu1
-
Trichoderma asperellum PQ34 X5DTB0 isoform Glu2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Trichoderma asperellum ?
-
 ?
carboxymethylcellulose + H2O
-
 Trichoderma asperellum PQ34 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, including 4 kDa signal peptide, calculated Trichoderma asperellum

Synonyms

Synonyms Comment Organism
Glu1
-
 Trichoderma asperellum
Glu2
-
 Trichoderma asperellum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Trichoderma asperellum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Trichoderma asperellum

Expression

Organism Comment Expression
Trichoderma asperellum highest expression occurs after 4 days of induction using 25 M/l methanol at 30°C when the yeast cells are cultured in a YPL medium additional information