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Literature summary for 3.2.1.4 extracted from
- Heterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris (2018), Int. J. Biol. Macromol., 106, 629-635 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Sclerotinia sclerotiorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sclerotinia sclerotiorum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant protein produced in Pichia pastoris has a molecular mass of 47 kDa, while native protein from Sclerotinia sclerotiorum has a mass of 34 kDa | Sclerotinia sclerotiorum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Pichia pastoris and native protein | Sclerotinia sclerotiorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19 | - |
recombinant protein, pH 7, 60°C | Sclerotinia sclerotiorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Sclerotinia sclerotiorum | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, native enzyme, x * 47000, recombinant enzyme expressed in Pichia pastoris | Sclerotinia sclerotiorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ENDO2 | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
native protein | Sclerotinia sclerotiorum |
| 60 | - |
recombinant protein | Sclerotinia sclerotiorum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
85% of maximum activity, recombinant protein | Sclerotinia sclerotiorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
native protein | Sclerotinia sclerotiorum |
| 7 | - |
recombinant protein | Sclerotinia sclerotiorum |
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Release 2023.1 (January 2023)
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