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Literature summary for 3.2.1.4 extracted from

  • Kanchanadumkerng, P.; Sakka, M.; Sakka, K.; Wiwat, C.
    Characterization of endoglucanase from Paenibacillus sp. M33, a novel isolate from a freshwater swamp forest (2017), J. Basic Microbiol., 57, 121-131 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus sp. M33

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 52% residual activity Paenibacillus sp. M33
Cu2+ 5 mM, 38% residual activity Paenibacillus sp. M33
EDTA 5 mM, 68% residual activity Paenibacillus sp. M33
Li+ 5 mM, 71% residual activity Paenibacillus sp. M33
Mg2+ 5 mM, 62% residual activity Paenibacillus sp. M33
Mn2+ 5 mM, 28% residual activity Paenibacillus sp. M33
NaCl 5 mM, 84% residual activity Paenibacillus sp. M33
SDS 0.5%, 60% residual activity Paenibacillus sp. M33
Triton X-100 0.5%, 73% residual activity Paenibacillus sp. M33
Zn2+ 5 mM, 51% residual activity Paenibacillus sp. M33

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. M33 A0A0N9GZK8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Paenibacillus sp. M33

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 substrate carboxymethylcellulose, pH 5.0, 50°C Paenibacillus sp. M33
0.14
-
 substrate barley beta-glucan, pH 5.0, 50°C Paenibacillus sp. M33
0.19
-
 substrate lichenan, pH 5.0, 50°C Paenibacillus sp. M33

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Paenibacillus sp. M33 ?
-
 ?
carboxymethylcellulose + H2O
-
 Paenibacillus sp. M33 ?
-
 ?
konjac glucomannan + H2O
-
 Paenibacillus sp. M33 ?
-
 ?
lichenan + H2O
-
 Paenibacillus sp. M33 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE and calculated Paenibacillus sp. M33

Synonyms

Synonyms Comment Organism
CelP
-
 Paenibacillus sp. M33

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus sp. M33

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, 80% residual activity Paenibacillus sp. M33

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Paenibacillus sp. M33

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10
-
 Paenibacillus sp. M33

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus sp. M33 calculated
-
 5