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Literature summary for 3.2.1.4 extracted from
- Characterization of a novel thermostable GH45 endoglucanase from Chaetomium thermophilum and its biodegradation of pectin (2017), J. Biosci. Bioeng., 124, 271-276 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermochaetoides thermophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | Km value 0.060 mg/ml, pH 4.0, 70°C | Thermochaetoides thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | V9PD44 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant protein expressed in Pichia pastoris | Thermochaetoides thermophila |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant protein | Thermochaetoides thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.52 | - |
substrate carboxymethylcellulose, pH 4.0, 70°C | Thermochaetoides thermophila |
| 2.14 | - |
substrate beta-1,4-D-glucan, pH 4.0, 70°C | Thermochaetoides thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-D-glucan + H2O | - |
Thermochaetoides thermophila | ? | - |
? |  |
| carboxymethylcellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
| pectin + H2O | - |
Thermochaetoides thermophila | galacturonic acid oligosaccharides | - |
? | |
| soluble starch + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE, x * 24500, calculated | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBH45-1 | - |
Thermochaetoides thermophila |
| CTendo45 | - |
Thermochaetoides thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, stabel | Thermochaetoides thermophila |
| 70 | - |
1 h, 79% residual activtiy | Thermochaetoides thermophila |
| 80 | - |
1 h, 66% residual activity | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Thermochaetoides thermophila |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermochaetoides thermophila | calculated | - |
5.9 |
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Release 2023.1 (January 2023)
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