Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Godoy, A.S.; Pereira, C.S.; Ramia, M.P.; Silveira, R.L.; Camilo, C.M.; Kadowaki, M.A.; Lange, L.; Busk, P.K.; Nascimento, A.S.; Skaf, M.S.; Polikarpov, I.
    Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members (2018), Sci. Rep., 8, 3678 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus niger Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.5 A resolution. Comparison between enzyme and other representative GH45 members Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
D114A complete loss of activity Phanerodontia chrysosporium
D85A about 10% of wild-type activity Phanerodontia chrysosporium
N92D replaxement of the general base in the catalytic mechanism, drastic decrease in activity. Unlike Asn92, residue Asp92 is mobile Phanerodontia chrysosporium
W154A about 50% of wild-type activity Phanerodontia chrysosporium
Y18A about 80% of wild-type activity Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
carboxymethylcellulose Km value 2 mg/mlpH 4.0, 70°C Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium B3Y002
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Phanerodontia chrysosporium ?
-
?
additional information no substrates: avicel, arabinoxylan and xyloglucan Phanerodontia chrysosporium ?
-
?

Synonyms

Synonyms Comment Organism
Cel45A
-
Phanerodontia chrysosporium
EgV
-
Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Phanerodontia chrysosporium