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Literature summary for 3.2.1.4 extracted from
- Thermodynamic and saccharification analysis of cloned GH12 endo-1,4-beta-glucanase from Thermotoga petrophila in a mesophilic host (2015), Protein Pept. Lett., 22, 785-794 .
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | its thermostability, resistance to heavy metal ions and specific activity make this enzyme an interesting candidate for industrial applications | Thermotoga petrophila |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli strain BL21 | Thermotoga petrophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, represses the enzyme activity up to 37% | Thermotoga petrophila |  |
| Hg2+ | 1 mM, represses the enzyme activity up to 22% | Thermotoga petrophila | |
| Mn2+ | 1 mM, represses the enzyme activity up to 40% | Thermotoga petrophila | |
| additional information | the enzyme displays optimal activity even in the existence of divalent and trivalent metallic cations: Ca2+, Mg2+, Pb2+, Cd2+, Cu2+, Ni2+ and Fe3+ | Thermotoga petrophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
carboxymethyl cellulose | pH 6.0, 95°C | Thermotoga petrophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Thermotoga petrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga petrophila | - |
- |
- |
| Thermotoga petrophila DSM 13995 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga petrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| barley beta-glucan + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
| laminarin + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| laminarin + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
| additional information | no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C | Thermotoga petrophila | ? | - |
? | |
| additional information | no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C | Thermotoga petrophila DSM 13995 | ? | - |
? | |
| Whatman filter paper + H2O | - |
Thermotoga petrophila | ? | - |
? | |
| Whatman filter paper + H2O | - |
Thermotoga petrophila DSM 13995 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GH12 endo-1,4-beta-glucanase | - |
Thermotoga petrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Thermotoga petrophila |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 50% of activity is found in a temperature range from 80°C to 100°C | Thermotoga petrophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
stable up to 8 h | Thermotoga petrophila |
| 97 | - |
half-life: 6.6 min | Thermotoga petrophila |
| 98 | - |
half-life: 2.73 min | Thermotoga petrophila |
| 99 | - |
half-life: 1.85 min | Thermotoga petrophila |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 209 | - |
carboxymethyl cellulose | pH 6.0, 80°C | Thermotoga petrophila | |
| 298 | - |
carboxymethyl cellulose | pH 6.0, 85°C | Thermotoga petrophila | |
| 356 | - |
carboxymethyl cellulose | pH 6.0, 90°C | Thermotoga petrophila | |
| 471 | - |
carboxymethyl cellulose | pH 6.0, 95°C | Thermotoga petrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
at 70°C | Thermotoga petrophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 50% of the retention of optimum rank of activity between pH 3.0 to pH 9.0 | Thermotoga petrophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
10 min, 20% of residual enzymatic activity | Thermotoga petrophila |
| 4 | - |
10 min, 50% of residual enzymatic activity is found at pH 4.0 to 7.0 | Thermotoga petrophila |
| 6 | - |
10 min,the recombinant enzyme is stable at pH 6.0 (citrate phosphate buffer) | Thermotoga petrophila |
| 7 | - |
10 min, 50% of residual enzymatic activity is found at pH 4.0 to 7.0 | Thermotoga petrophila |
| 10 | - |
10 min, 20% of residual enzymatic activity | Thermotoga petrophila |
| 11 | - |
10 min, 20% of residual enzymatic activity | Thermotoga petrophila |
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