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Literature summary for 3.2.1.4 extracted from

  • Saxena, H.; Hsu, B.; de Asis, M.; Zierke, M.; Sim, L.; Withers, S.G.; Wakarchuk, W.
    Characterization of a thermostable endoglucanase from Cellulomonas fimi ATCC484 (2018), Biochem. Cell Biol., 96, 68-76 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene Celf_1230, DNA and amino acid sequence determination and analysis, sequence comparisons, codon-optimized, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli Cellulomonas fimi
gene Celf_3184, sequence comparisons, recombinant expression Cellulomonas fimi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis Cellulomonas fimi

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Cellulomonas fimi
-
-

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi F4GZY2
-
-
Cellulomonas fimi F4H413
-
-
Cellulomonas fimi ATCC 484 F4GZY2
-
-
Cellulomonas fimi ATCC 484 F4H413
-
-
Cellulomonas fimi DSM 20113 F4GZY2
-
-
Cellulomonas fimi DSM 20113 F4H413
-
-
Cellulomonas fimi JCM 1341 F4GZY2
-
-
Cellulomonas fimi JCM 1341 F4H413
-
-
Cellulomonas fimi NBRC 15513 F4GZY2
-
-
Cellulomonas fimi NBRC 15513 F4H413
-
-
Cellulomonas fimi NCIMB 8980 F4GZY2
-
-
Cellulomonas fimi NCIMB 8980 F4H413
-
-
Cellulomonas fimi NCTC 7547 F4GZY2
-
-
Cellulomonas fimi NCTC 7547 F4H413
-
-

Purification (Commentary)

Purification (Comment) Organism
gene recombinant N-terminally His-tagged enzyme from Escherichia coli by ultracentrifugation at 100000 x g, nickel affinity chromatography, and ultrafiltration Cellulomonas fimi
recombinant enzyme Cellulomonas fimi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi NBRC 15513 ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi NCIMB 8980 ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi NCTC 7547 ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi JCM 1341 ?
-
 ?
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Cellulomonas fimi DSM 20113 ?
-
 ?
2,4-dinitrophenyl beta-cellotrioside + H2O cleavage occurs between the second and third sugar residues with no release of chromophore, thus no colour change Cellulomonas fimi 2,4-dinitrophenyl beta-cellobioside + D-glucose
-
 ?
6-chloro-4-methylumbelliferyl beta-cellobioside + H2O
-
 Cellulomonas fimi 6-chloro-4-methylumbelliferone + cellobiose
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NBRC 15513 ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NCIMB 8980 ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NCTC 7547 ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi ATCC 484 ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi JCM 1341 ?
-
 ?
barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi DSM 20113 ?
-
 ?
cellohexaose + H2O
-
 Cellulomonas fimi cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi NBRC 15513 cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi NCIMB 8980 cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi NCTC 7547 cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi ATCC 484 cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi JCM 1341 cellotriose + cellobiose
-
 ?
cellopentaose + H2O
-
 Cellulomonas fimi DSM 20113 cellotriose + cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi NBRC 15513 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi NCIMB 8980 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi NCTC 7547 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi ATCC 484 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi JCM 1341 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Cellulomonas fimi DSM 20113 2 cellobiose
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NBRC 15513 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NBRC 15513 ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NCIMB 8980 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NCIMB 8980 ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NCTC 7547 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NCTC 7547 ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi ATCC 484 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi ATCC 484 ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi JCM 1341 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi JCM 1341 ?
-
 ?
additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi DSM 20113 ?
-
 ?
additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi DSM 20113 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi NBRC 15513 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi NCIMB 8980 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi NCTC 7547 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi JCM 1341 ?
-
 ?
phosphoric acid swollen cellulose + H2O
-
 Cellulomonas fimi DSM 20113 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi NBRC 15513 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi NCIMB 8980 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi NCTC 7547 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi JCM 1341 ?
-
 ?
tamarind xyloglucan + H2O
-
 Cellulomonas fimi DSM 20113 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45450, about, sequence calculation Cellulomonas fimi
? x * 48250, about, sequence calculation Cellulomonas fimi

Synonyms

Synonyms Comment Organism
Celf_1230
-
 Cellulomonas fimi
Celf_3184
-
 Cellulomonas fimi
CfCel6A
-
 Cellulomonas fimi
CfCel6C
-
 Cellulomonas fimi
endoglucanase
-
 Cellulomonas fimi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Cellulomonas fimi
65
-
-
 Cellulomonas fimi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 74 activity range, low activity at 30°C, inactivation at 74°C Cellulomonas fimi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 Tm of enzyme CfCel6C Cellulomonas fimi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4260
-
 phosphoric acid swollen cellulose pH 6.0, 50°C, recombinant enzyme Cellulomonas fimi
26100
-
 phosphoric acid swollen cellulose pH 5.5, 65°C, recombinant enzyme Cellulomonas fimi
128000
-
 Barley beta-glucan pH 6.0, 50°C, recombinant enzyme Cellulomonas fimi
295000
-
 Barley beta-glucan pH 5.5, 65°C, recombinant enzyme Cellulomonas fimi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Cellulomonas fimi
6
-
-
 Cellulomonas fimi

General Information

General Information Comment Organism
additional information the enzyme has no recognizable carbohydrate binding module, but a glycoside hydrolase family 6 domain Cellulomonas fimi