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Literature summary for 3.2.1.4 extracted from
- Biochemical and functional characterization of a novel thermoacidophilic, heat and halo-ionic liquids tolerant endo-beta-1,4-glucanase from saline-alkaline lake soil microbial metagenomic DNA (2018), Int. J. Biol. Macromol., 118, 1035-1044 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
uncultured bacterium |  |
| DTT | - |
uncultured bacterium | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene nmGH45, cloned from saline-alkaline lake soil microbial metagenomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Pichia pastoris strain GS115, co-expression of molecular chaperone BIP enhances enzyme protein secretion by 20%, but chaperones PDI and HAC1 have limited influences | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butyl-3-methylimidazolium chloride | 92.8% and 43.8% activity remaining in 10% and 20% 1-butyl-3-methylimidazolium chloride | uncultured bacterium | |
| Ca2+ | slight inhibition | uncultured bacterium | |
| EDTA | slight inhibition | uncultured bacterium | |
| Pb2+ | slight inhibition | uncultured bacterium | |
| SDS | strong inhibition | uncultured bacterium | |
| Triton X-100 | slight inhibition | uncultured bacterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates slightly | uncultured bacterium | |
| Cu2+ | activates | uncultured bacterium | |
| Mn2+ | activates | uncultured bacterium | |
| additional information | no or poor effects by Li+, K+, Na+, Mg2+, and Fe3+ | uncultured bacterium | |
| NaCl | enzyme NMgh45 retains 88% activity in 4 M NaCl after 24 h | uncultured bacterium | |
| Ni2+ | activates slightly | uncultured bacterium | |
| Zn2+ | activates | uncultured bacterium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | A0A286Q5D2 | isolated from saline-alkaline lake soil | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain GS115 | uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.13 | - |
purified recombinant enzyme, pH 5.0, 60°C, substrate laminarin | uncultured bacterium |
| 10.9 | - |
purified recombinant enzyme, pH 5.0, 60°C, substrate Avicel PH-101 | uncultured bacterium |
| 65.53 | - |
purified recombinant enzyme, pH 5.0, 60°C, substrate carboxymethyl cellulose | uncultured bacterium |
| 92.29 | - |
purified recombinant enzyme, pH 5.0, 60°C, substrate barley beta-glucan | uncultured bacterium |
| 134.22 | - |
purified recombinant enzyme, pH 5.0, 60°C, substrate lichenin | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Avicel PH-101 + H2O | low activity | uncultured bacterium | ? | - |
? | |
| barley beta-glucan + H2O | moderate activity | uncultured bacterium | ? | - |
? | |
| carboxymethyl cellulose + H2O | moderate activity | uncultured bacterium | ? | - |
? | |
| laminarin + H2O | low activity | uncultured bacterium | ? | - |
? | |
| lichenin + H2O | best substrate | uncultured bacterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22500, about, sequence calculation, x * 37000, recombinant enzyme, SDS-PAGE | uncultured bacterium |
| More | modeled tertiary structure of NMgh45, overview | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-glucanase | - |
uncultured bacterium |
| nmGH45 | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | - |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | over 60% of maximal activity within this range, profile overview | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | recombinant enzyme NMgh45, completely stable for 2 h | uncultured bacterium |
| 80 | - |
recombinant enzyme NMgh45, 90% activity remains after 2 h | uncultured bacterium |
| 90 | - |
recombinant enzyme NMgh45, 68% activity remains after 1 h, 40% after 2 h | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | and below, over 50% of maximal activity within this range, profile overview | uncultured bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | recombinant enzyme NMgh45, over 80% activity remains after 24 h | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 45, GH45 | uncultured bacterium |
| additional information | compared with other non-halotolerant counterparts, enzyme NMgh45 is enriched with acidic amino acids on the protein surface, and the conformation of NMgh45 is stable at high salinity condition, three-dimensional structure, molecular modeling and dynamic simulations using the structures with PDB IDs 1L8F, 2ENG, and 5GLX_A as the templates, overview | uncultured bacterium |
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