Literature summary for 3.2.1.4 extracted from

Kim, E.S.; Kim, B.S.; Kim, K.Y.; Woo, H.M.; Lee, S.M.; Um, Y.
Aerobic and anaerobic cellulose utilization by Paenibacillus sp. CAA11 and enhancement of its cellulolytic ability by expressing a heterologous endoglucanase (2018), J. Biotechnol., 268, 21-27 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene 168cel5 or eglS, recombinant expression of the enzyme, in an expression construct comprising DNA sequences encoding P43 promoter, signal peptide of Bacillus subtilis nprB, and mature Bacillus subtilis strain 168 Cel5 residues 30-499, in Paenibacillus sp. strain CAA11	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
additional information	cellulolytic activity of Paenibacillus sp. strain CAA11 is significantly enhanced by expressing a heterologous endoglucanase 168Cel5 from Bacillus subtilis (DNA sequences encoding P43 promoter, signal peptide of Bacillus subtilis nprB, and mature Bacillus subtilis strain 168 Cel5 residues 30-499) under both aerobic and anaerobic conditions. The strain harboring the 168cel5 gene reveals 2fold bigger halo zone on Congo-red plate and 1.75fold more aerobic cellulose utilization in liquid medium compared with the negative control. Under anaerobic conditions, the recombinant strain expressing 168Cel5 consumes 1.83fold more cellulose (5.10 g/l) and produces 5fold more ethanol (0.65 g/l) along with 5fold more total acids (1.6 g/l) compared with the control, resulting in 2.73fold higher yields. Optimal growth of the engineered strain at 37°C, evaluation of optimal growth conditions, overview	Paenibacillus sp. CAA11
additional information	cellulolytic activity of Paenibacillus sp. strain CAA11 is significantly enhanced by expressing a heterologous endoglucanase 168Cel5 from Bacillus subtilis under both aerobic and anaerobic conditions. The strain harboring the 168cel5 gene reveals 2fold bigger halo zone on Congo-red plate and 1.75fold more aerobic cellulose utilization in liquid medium compared with the negative control. Under anaerobic conditions, the recombinant strain expressing 168Cel5 consumes 1.83fold more cellulose (5.10 g/l) and produces 5fold more ethanol (0.65 g/l) along with 5fold more total acids (1.6 g/l) compared with the control, resulting in 2.73fold higher yields	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Bacillus subtilis	-	-
extracellular	the enzyme is secreted	Paenibacillus sp. CAA11	-	-

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P10475	-	-
Bacillus subtilis 168	P10475	-	-
Paenibacillus sp. CAA11	A0A2S0UGE4	isolated from sediment	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	Paenibacillus sp. strain CAA11 is able to utilize cellulose as a sole carbon source	Paenibacillus sp. CAA11	-

Synonyms

Synonyms	Comment	Organism
168cel5	-	Bacillus subtilis
DCC85_10145	locus name	Paenibacillus sp. CAA11
EglS	-	Bacillus subtilis

General Information

General Information	Comment	Organism
physiological function	Paenibacillus sp. strain CAA11 secretes cellulolytic enzymes by its own secretion system and produces ethanol as well as short-chain organic acids (formic acid, acetic acid, lactic acid) from cellulose with rel. low activity	Paenibacillus sp. CAA11

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Release 2023.1 (January 2023)