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Literature summary for 3.2.1.4 extracted from
- Expression and characterisation of a thermophilic endo-1,4-beta-glucanase from Sulfolobus shibatae of potential industrial application (2018), Mol. Biol. Rep., 45, 2201-2211 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the thermophilic nature and biochemical properties of the enzyme indicate its potential suitability in industrial applications undertaken at high temperature, such as the production of second-generation bioethanol from lignocellulosic feedstocks and in the brewing industry | Saccharolobus shibatae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ssgluc, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B (DE3), subcloning in Escherichia coli strain DH5alpha | Saccharolobus shibatae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | A0A1B5G0A1 | - |
- |
| Saccharolobus shibatae B12 | A0A1B5G0A1 | - |
- |
| Saccharolobus shibatae DSM 5389 | A0A1B5G0A1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His-tagged enzyme 2535fold from Escherichia coli by nickel affinity chromatography and ultrafiltration | Saccharolobus shibatae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 428 | - |
purified recombinant His-tagged enzyme, pH 4.0, 95°C, substrate carboxymethyl cellulose | Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | best substrate | Saccharolobus shibatae | ? | - |
? |  |
| barley beta-glucan + H2O | best substrate | Saccharolobus shibatae B12 | ? | - |
? | |
| barley beta-glucan + H2O | best substrate | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae B12 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| lichenan + H2O | best substrate | Saccharolobus shibatae | ? | - |
? | |
| lichenan + H2O | best substrate | Saccharolobus shibatae B12 | ? | - |
? | |
| lichenan + H2O | best substrate | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae | ? | - |
? | |
| additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae B12 | ? | - |
? | |
| additional information | the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C | Saccharolobus shibatae DSM 5389 | ? | - |
? | |
| xylan + H2O | - |
Saccharolobus shibatae | ? | - |
? | |
| xylan + H2O | - |
Saccharolobus shibatae B12 | ? | - |
? | |
| xylan + H2O | - |
Saccharolobus shibatae DSM 5389 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35128, sequence calculation | Saccharolobus shibatae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-B-glucanase | UniProt | Saccharolobus shibatae |
| endo-1,4-beta-glucanase | - |
Saccharolobus shibatae |
| endo-beta-glucanase | - |
Saccharolobus shibatae |
| endoglucanase | - |
Saccharolobus shibatae |
| ssgluc | - |
Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 100 | recombinant enzyme | Saccharolobus shibatae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 110 | recombinant enzyme, over 20% of maximal activity within this range, 50% at 80°C and 105°C, profile overview | Saccharolobus shibatae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
purified recombinant enzyme, completely stable for 1 h, and 98% activity remaining after 2 h | Saccharolobus shibatae |
| 80 | - |
purified recombinant enzyme, completely stable for 1 h, and 90% activity remaining after 2 h | Saccharolobus shibatae |
| 85 | - |
purified recombinant enzyme, completely stable for 1 h, and 84% activity remaining after 2 h | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
recombinant enzyme | Saccharolobus shibatae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | recombinant enzyme, over 91% of maximal activity within this range, pH profile, overview | Saccharolobus shibatae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus shibatae | sequence calculation | - |
4.98 |
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