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Literature summary for 3.2.1.40 extracted from
- Molecular identification of an alpha-L-rhamnosidase from Bacillus sp strain GL1 as an enzyme involved in complete metabolism of gellan (2003), Arch. Biochem. Biophys., 415, 235-244.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 1 mM, 4% residual activity; 1 mM, 70% residual activity | Bacillus sp. (in: Bacteria) |  |
| D-glucose | 10 mM, 56% residual activity | Bacillus sp. (in: Bacteria) | |
| FeCl3 | 1 mM, 76% residual activity | Bacillus sp. (in: Bacteria) | |
| HgCl2 | 0.1 mM, 0.23% residual activity | Bacillus sp. (in: Bacteria) | |
| L-rhamnose | 10 mM, 14% residual activity; 10 mM, 37% residual activity | Bacillus sp. (in: Bacteria) | |
| p-chloromercuribenzoic acid | 0.1 mM, 50% residual activity | Bacillus sp. (in: Bacteria) | |
| ZnCl2 | 1 mM, 73% residual activity | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.119 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | pH 7.0 | Bacillus sp. (in: Bacteria) | |
| 0.282 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | pH 7.0 | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 98000 | - |
5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 98300 | - |
5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 100000 | - |
gel filtration, PAGE | Bacillus sp. (in: Bacteria) |
| 105000 | - |
1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 106000 | - |
1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 500000 | - |
gel filtration, PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus sp. (in: Bacteria) | isozyme plays a crucial role in complete metabolism of gellan | ? | - |
? | |
| additional information | Bacillus sp. (in: Bacteria) GL1 | isozyme plays a crucial role in complete metabolism of gellan | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | Q93RE7 | ene rhaB, when grown on gellan medium, only this isoform is expressed | - |
| Bacillus sp. (in: Bacteria) | Q93RE8 | gene rhaA | - |
| Bacillus sp. (in: Bacteria) GL1 | Q93RE7 | ene rhaB, when grown on gellan medium, only this isoform is expressed | - |
| Bacillus sp. (in: Bacteria) GL1 | Q93RE8 | gene rhaA | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | - |
Bacillus sp. (in: Bacteria) |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 54 | - |
pH 7.0 | Bacillus sp. (in: Bacteria) |
| 54.4 | - |
pH 7.0 | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gellan + H2O | - |
Bacillus sp. (in: Bacteria) | L-rhamnose + ? | - |
? | |
| gellan + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | L-rhamnose + ? | - |
? | |
| additional information | isozyme plays a crucial role in complete metabolism of gellan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| additional information | isozyme plays a crucial role in complete metabolism of gellan | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? | |
| naringin + H2O | - |
Bacillus sp. (in: Bacteria) | L-rhamnose + ? | - |
? | |
| naringin + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | L-rhamnose + ? | - |
? | |
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + beta-D-fucopyranose | 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
| p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | p-nitrophenol + beta-D-fucopyranose | 2.9% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
| p-nitrophenyl-beta-D-galactopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | p-nitrophenol + beta-D-galactopyranose | 1.6% of activity compared to p-nitrophenyl-alpha-L-rhamnopyranoside | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 106000, deduced from gene sequence, 1 * 105000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| pentamer | 5 * 98300, deduced from gene sequence, 5 * 98000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Bacillus sp. (in: Bacteria) |
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