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Literature summary for 3.2.1.40 extracted from
- Purification and characterization of an alpha-L-rhamnosidase from Aspergillus terreus of interest in winemaking. (2001), J. Food Sci., 66, 204-209.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Aspergillus terreus |  |
| D-glucose | 21% w/v, decrease in activity by 20% | Aspergillus terreus | |
| ethanol | 12% v/v, decrease in activity by 20% | Aspergillus terreus | |
| Hg2+ | - |
Aspergillus terreus | |
| L-rhamnose | competitive | Aspergillus terreus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | - |
Aspergillus terreus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulation | Aspergillus terreus | |
| Co2+ | stimulation | Aspergillus terreus | |
| Mg2+ | stimulation | Aspergillus terreus | |
| Zn2+ | stimulation | Aspergillus terreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96000 | - |
x * 96000, SDS-PAGE | Aspergillus terreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terreus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus terreus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | culture condition: L-rhamnose as sole carbon source, enzyme biosynthesis is repressed by glucose | Aspergillus terreus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Aspergillus terreus | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 96000, SDS-PAGE | Aspergillus terreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Aspergillus terreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Aspergillus terreus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
L-rhamnose | - |
Aspergillus terreus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus terreus | isoelectric focusing | - |
4.9 |
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