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Literature summary for 3.2.1.41 extracted from
- Extracellular and protease-released pullulanases (1976), J. Bacteriol., 125, 501-508.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cyclodextrins | - |
Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | 0.078 | pullulan | glucose equivalents | Klebsiella aerogenes |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | protease-released | Klebsiella aerogenes | 5737 | - |
| extracellular | - |
Klebsiella aerogenes | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126000 | - |
protease-released enzyme, gel filtration | Klebsiella aerogenes |
| 141000 | - |
extracellular enzyme, gel filtration | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Klebsiella aerogenes | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Klebsiella aerogenes | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4 months, 4°C | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Klebsiella aerogenes | maltotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 141000, gel electrophoresis | Klebsiella aerogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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