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Literature summary for 3.2.1.41 extracted from
- Purification, crystallization and some properties of intracellular pullulanase from Aerobacter aerogenes (1973), Agric. Biol. Chem., 37, 2821-2826.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation | Klebsiella aerogenes |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Klebsiella aerogenes | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | 90000 | gel filtration, sedimentation equilibrium | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Klebsiella aerogenes | branched polysaccharides | maltooligosaccharides + maltose | - |
? |  |
| pullulan + H2O | Klebsiella aerogenes | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella aerogenes | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Klebsiella aerogenes | maltotriose + ? | - |
? | |
| soluble starch | - |
Klebsiella aerogenes | maltotriose + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Klebsiella aerogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Klebsiella aerogenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pullulan | Klebsiella aerogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
instable above | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Klebsiella aerogenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 12 | - |
Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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