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Literature summary for 3.2.1.41 extracted from
- Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023 (1989), Mol. Microbiol., 4, 73-85.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Klebsiella pneumoniae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme exits as an cell-surface-bound intermediate prior release into the medium | Klebsiella pneumoniae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | 116000 | mature protein | Klebsiella pneumoniae |
| 118000 | - |
precursor protein, gel electrophoresis, amino acid analysis | Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Klebsiella pneumoniae | branched polysaccharides | maltooligosaccharides + maltose | - |
? |  |
| amylopectin + H2O | Klebsiella pneumoniae UNF5023 | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Klebsiella pneumoniae | - |
maltotriose + ? | - |
? | |
| pullulan + H2O | Klebsiella pneumoniae UNF5023 | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
UNF5023 | - |
| Klebsiella pneumoniae UNF5023 | - |
UNF5023 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Klebsiella pneumoniae | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella pneumoniae | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | - |
Klebsiella pneumoniae UNF5023 | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Klebsiella pneumoniae UNF5023 | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Klebsiella pneumoniae | maltotriose + ? | - |
? | |
| pullulan + H2O | - |
Klebsiella pneumoniae UNF5023 | maltotriose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type I | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan | Klebsiella pneumoniae |
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