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Literature summary for 3.2.1.41 extracted from
- Purification and biochemical characterization of pullulanase type I from Thermus caldophilus GK-24 (1996), FEMS Microbiol. Lett., 138, 147-152.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Thermus caldophilus |  |
| Cyclodextrins | - |
Thermus caldophilus | |
| Fe2+ | - |
Thermus caldophilus | |
| Zn2+ | - |
Thermus caldophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Thermus caldophilus | |
| Mg2+ | - |
Thermus caldophilus | |
| Mn2+ | - |
Thermus caldophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration, SDS-PAGE | Thermus caldophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Thermus caldophilus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Thermus caldophilus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus caldophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Thermus caldophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermus caldophilus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Thermus caldophilus | maltooligosaccharides + maltose | - |
? | |
| dextrin + H2O | - |
Thermus caldophilus | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Thermus caldophilus | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Thermus caldophilus | maltotriose + ? | - |
? | |
| soluble starch | - |
Thermus caldophilus | maltotriose + maltose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000 | Thermus caldophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type I | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan | Thermus caldophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermus caldophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
after 30 min, pH 5.5, no loss of activity | Thermus caldophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermus caldophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | stable at 4°C for 24 h | Thermus caldophilus |
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