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Literature summary for 3.2.1.41 extracted from
- Heat-stable pullulanase from Bacillus acidopullulyticus: characterization and refolding after guanidinium chloride-induced unfolding (1999), Extremophiles, 3, 147-152.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | assists reactivation after unfolding | Bacillus acidopullulyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Guanidine HCl | unfolding of the enzyme | Bacillus acidopullulyticus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances stability | Bacillus acidopullulyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
gel filtration, SDS-PAGE | Bacillus acidopullulyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Bacillus acidopullulyticus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Bacillus acidopullulyticus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus acidopullulyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus acidopullulyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | promozym 200L | Bacillus acidopullulyticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Bacillus acidopullulyticus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Bacillus acidopullulyticus | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Bacillus acidopullulyticus | maltotriose + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 5.5, after 60 min 55% loss of activity | Bacillus acidopullulyticus |
| 60 | - |
sucrose, polyols, Na2SO4 stabilze enzyme | Bacillus acidopullulyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
91% loss of activity at 60°C for 30 min | Bacillus acidopullulyticus |
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