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Literature summary for 3.2.1.41 extracted from
- Characterization of pullulan-hydrolysing enzyme from an alkalopsychotropic Micrococcus sp. (1990), Appl. Microbiol. Biotechnol., 34, 52-56.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Micrococcus sp. |  |
| Cu2+ | - |
Micrococcus sp. | |
| Cyclodextrins | - |
Micrococcus sp. | |
| EDTA | - |
Micrococcus sp. | |
| Hg2+ | - |
Micrococcus sp. | |
| Mn2+ | - |
Micrococcus sp. | |
| N-bromosuccinimide | at 5 mM | Micrococcus sp. | |
| Ni2+ | - |
Micrococcus sp. | |
| Pb2+ | - |
Micrococcus sp. | |
| Sn2+ | - |
Micrococcus sp. | |
| Sodium dodecyl sulfate | - |
Micrococcus sp. | |
| Zn2+ | - |
Micrococcus sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Micrococcus sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances stability | Micrococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
SDS-PAGE | Micrococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Micrococcus sp. | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Micrococcus sp. | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Micrococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Micrococcus sp. | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Micrococcus sp. | maltotriose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type I | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan | Micrococcus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Micrococcus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | - |
Micrococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Micrococcus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Micrococcus sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 10% loss of activity in the presence of CaCl2 | Micrococcus sp. |
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